Micromass UK Limited

Leco Corporation

Waters Technologies Corporation

Waters Technologies Ireland Limited

Keith George Richardson

Jason Lee Wildgoose

Martin Raymond Green

Steven Derek Pringle

Kevin Giles

David John Langridge

Michael Raymond Morris

Zoltan Takats

Emrys Jones

Julia Balog

Jeffery Mark Brown

James Ian Langridge

Daniel Szalay

Lajos Godorhazy

Daniel Simon

John Brian Hoyes

Richard Denny

Daniel James Kenny

Paul Robert Murray

Tamas Karancsi

Frances Bolt

Anatoly Verenchikov

Mikhail Yavor

John Skilling

Ian Sinclair

David Darrell Williams

Richard Barrington Moulds

Richard Chapman

Stephen John Platt

Pamela Pruski

Phillip Robert Bennett

David Alan MacIntyre

Robert Harold Bateman

Anthony James Gilbert

Tamás Karancsi

Therese McKenna

Zoltán Takáts

Emmy Maria Hoyes

Iain David Grant Campuzano

David Gordon

Emmanuelle Claude

Boris Kozlov

Garry Michael Scott

Kate Whyatt

Peter Gerard Spreadbury

Hans Vissers

Farnoush Salarzaei

Jakub Ujma

Peter Nixon

Mike Morris

Johannes Petrus Cornelis Vissers

Marten F Snel

Sandra Richardson

Vasiliy Makarov

A method of mass spectrometry is disclosed comprising: a step () of analysing a reference compound in a first mass spectrometer and outputting mass spectral data in response thereto; a step () of analysing the reference compound in a second, different mass spectrometer and outputting mass spectral data in response thereto; and a step () of automatically adjusting an operational parameter, duty cycle (e.g. duty cycle of data acquisition), or acquired spectral data of at least one mass spectrometer such that, for the same (given) consumption of reference compound by the spectrometer, the statistical precision of quantification (the number of detected ions) and/or of mass measurement (the mass resolution) by the mass spectrometer is substantially the same as that of the other mass spectrometer. A similar method of ion mobility spectrometry is disclosed.

Automatically standardising spectrometers

A method of analysis using mass and/or ion mobility spectrometry or ion mobility spectrometry is disclosed comprising: using a first device to generate aerosol, smoke or vapour from one or more regions of a first target of biological material; and mass and/or ion mobility analysing and/or ion mobility analysing said aerosol, smoke, or vapour, or ions derived therefrom so as to obtain first spectrometric data. The method may use an ambient ionisation method.

Tissue analysis by mass spectrometry or ion mobility spectrometry

Disclosed herein are various methods and apparatus for performing charge detection mass spectrometry (CDMS). In particular, techniques are disclosed for monitoring a detector signal from a CDMS device to determine how many ions are present in the ion trap () of the CDMS device. For example, if no ions are present the measurement can then be terminated early. Similarly, if more than one ion is present, the measurement can be terminated early, or ions can be removed from the trap () until only a single ion remains. Techniques are also provided for increasing the probability of there being a single ion in the trap (). A technique for attenuating an ion beam is also provided.

Charge detection mass spectrometry

A method of correcting mass spectral data comprises making calibration measurements of first intrinsic components (A, B, C) at one or more calibration times (t1) using calibrants which have known mass to charge ratio (m/z) values or previously mass measured mass to charge ratio (m/z) values, making a list of second intrinsic components (D, E, F) which are present during more than one acquisition periods, wherein the second intrinsic components have mass to charge ratio (m/z) values that were not present or observed during or close to the one or more calibration times (t1) but which do overlap in time with the first intrinsic components (A, B, C), and utilising the list to calculate a mass or mass to charge ratio (m/z) correction factor for one or more acquisition periods which are not close or adjacent in time to an acquisition period containing a directly calibrated mass to charge ratio (m/z) value.

Adaptive intrinsic lock mass correction

A method is disclosed comprising: trapping ions in an ion trap (); applying a first force on the ions within the ion trap in a first direction, said force having a magnitude that is dependent upon the value of a physicochemical property of the ions; applying a second force on these ions in the opposite direction so that the ions separate according to the physicochemical property value as a result of the first and second forces; and then pulsing or driving ions out of one or more regions of the ion trap.

Separating ions in an ion trap

A method of mass spectrometry is disclosed comprising: performing a plurality of cycles of operation during a single experimental run, wherein each cycle comprises: mass selectively transmitting precursor ions of a single mass, or range of masses, through or out of a mass separator or mass filter at any given time, wherein the mass separator or mass filter is operated such that the single mass or range of masses transmitted therefrom is varied with time; and mass analysing ions.

Two dimensional MSMS

A method of mass spectrometry is disclosed comprising: a) providing temporally separated precursor ions; b) mass analyzing separated precursor ions, and/or product ions derived therefrom, during a plurality of sequential acquisition periods, wherein the value of an operational parameter of the spectrometer is varied during the different acquisition periods; c) storing the spectral data obtained in each acquisition period along with its respective value of the operational parameter; d) interrogating the stored spectral data and determining which of the spectral data for a precursor ion or product ions meets a predetermined criterion, and determining the value of the operational parameter that provides this mass spectral data as a target operational parameter value; and e) mass analyzing again the precursor or product ions whilst the operational parameter is set to the target operational parameter value.

Optimised targeted analysis

A method of self-calibrating a mass spectrometer or mass spectral data is disclosed. At least some first observed mass to charge ratios are matched with or against a comprehensive reference set of possible or predicted elemental compositions having known precise mass to charge ratios. One or more calibration parameters of a calibration routine are then adjusted so as to optimise the match between one or more of the first observed mass to charge ratios and the corresponding known precise mass to charge ratios of one or more possible or predicted elemental compositions contained within the reference set.

Self-calibration of high resolution mass spectrum

A method of encoding a parent or precursor ion beam to aid product ion assignment is disclosed. According to an embodiment the energy of parent ions entering a collision cellis progressively increased. Different species of parent ions fragment at different collision energies. Fragment ion intensity profiles are matched with parent ion intensity profiles to correlate fragment ions with corresponding parent ions.

Encoding of precursor ion beam to aid product ion assignment

A method of analysis using mass spectrometry and/or ion mobility spectrometry is disclosed. The method comprises: using a first device to generate smoke, aerosol or vapour from a target comprising or consisting of a microbial population; mass analysing and/or ion mobility analysing said smoke, aerosol or vapour, or ions derived therefrom, in order to obtain spectrometric data; and analysing said spectrometric data in order to analyse said microbial population.

Spectrometric analysis of microbes

A method of analysis using mass spectrometry and/or ion mobility spectrometry is disclosed comprising: (a) using a first device to generate smoke, aerosol or vapour from a target in vitro or ex vivo cell population; (b) mass analysing and/or ion mobility analysing said smoke, aerosol or vapour, or ions derived therefrom, in order to obtain spectrometric data; and (c) analysing said spectrometric data in order to identify and/or characterise said target cell population or one or more cells and/or compounds present in said target cell population.

Cell population analysis

Growing community of inventors

High Peak, United Kingdom

Keith George Richardson