Other

Bio-rad Laboratories, Inc.

Jumpcode Genomics, Inc.

Igenomx International Genomics Corporation

The Scripps Research Institute

Raindance Technologies, Inc.

Keith Brown

Darren Roy Link

Michael L Samuels

Jeffrey Charles Olson

Andrew Watson

Peter Dansky

Michael S Zelten

Daniel R Salomon

Steven Robert Head

Phillip T Ordoukhanian

Ben Grizzard

Bennett C Grizzard, Jr

Azeem Siddique

The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

Compositions and methods for molecular labeling

Disclosed herein are compositions and methods related to the elimination of molecules of a selected sequence from a nucleic acid sample or from an sequence dataset resulting from the sequencing of a sample, for example to exclude such molecules from downstream analysis or sequencing, or to exclude such sequences from a downstream data set.

Genome fractioning

Disclosed herein are methods of long range target specific amplification and sequencing using an RNA intermediate synthesized directly from the target including using hairpin adaptors having a double stranded promoter and an overhang which hybridizes with a reverse-complementary overhang on a target nucleic acid. RNA transcription eliminates clonal amplification of early synthesis errors. Approaches allow for the identification of target-adjacent sequence, such as sequence adjacent to a repeat element target. Also disclosed herein are compositions and kits for amplification and sequencing.

Method for target specific RNA transcription of DNA sequences

Rapid nucleic acid libraries, methods of generation, kits, and compositions relating to library synthesis, including reagents, intermediaries and final products are disclosed herein. The disclosure enables rapid synthesis of libraries that allow independent verification of sequence information and rapid identification of sequence information with template of origin.

Methods and compositions for rapid nucleic acid library preparation

Methods, compositions and kits are provided herein for insertional modification of nucleic acids by, for example transposase-mediated covalent insertion of insertion sequence into a sample nucleic acid molecule. Using sequence of the insertion to direct amplification of adjacent nucleic acid sequence, and using bar codes to map amplified sequence to partitions, one can map sample nucleic acid sequence to single molecules of the nucleic acid sample that are derived directly from the sample nucleic acid molecule.

Methods and compositions for sequencing

Growing community of inventors

Carlsbad, CA, United States of America

Keith Brown