Roche Molecular Systems, Inc.

Cea/institut De Genomique—centre National De Genotypage

Roche Medical Systems, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Keith Bauer

Thomas W Myers

Fred Lawrence Reichert

Shawn Lee Suko

David H Gelfand

Joseph San Filippo

Nancy J Schoenbrunner

Ellen H Fiss

Edward Soh Smith

Rachel Shahinian

Amar Gupta

Veeraiah Bodepudi

Susanne Stoffel

Barbara Eckert

Ivo Glynne Gut

John Niemiec

Florence Mauger

Olga Budker

Thomas W Meyer

Shawn Suko

Disclosed are DNA polymerases having improved ability to incorporate methylated-dNTPs, relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the DNA polymerases. Further disclosed are kits and reaction mixtures comprising the improved DNA polymerases as well as methods of primer extension using the improved DNA polymerases.

DNA polymerases for efficient and effective incorporation of methylated-dNTPS

Disclosed are mutant DNA polymerases having increased 3'-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.

DNA polymerases with increased 3'-mismatch discrimination

Disclosed are DNA polymerases having increased reverse transcriptase efficiency, mismatch tolerance, extension rate and/or tolerance of RT and polymerase inhibitors relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the DNA polymerases.

DNA polymerases with improved activity

Provided are DNA polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of primer extension methods. Also provided are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the DNA polymerases.

Disclosed are DNA polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the DNA polymerases.

The present application provides polynucleotides comprising 5'-tails with sequence segments useful for the detection of target nucleic acid sequences, and methods for their use in detecting target nucleic acids. The polynucleotides are used to amplify a subsequence of a target nucleic acid in the presence of one or more ribonucleotides. The ribonucleotides are incorporated into amplification products at regular intervals complementary to the 5&#x2032;-tail sequence segments. Cleavage of amplification products at the bond immediately 3&#x2032; to incorporated ribonucleotides produces detectably distinct fragments indicative of the presence or absence of a target nucleic acid.

Ribonucleotide tag nucleic acid detection

Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.

DNA polymerases and related methods

Growing community of inventors

San Rafael, CA, United States of America

Keith Bauer