The University of Tokyo

Nissan Chemical Industries Limited

Linkbio Co., Ltd.

Japan Science and Technology Agency

Keitaro Yoshimoto

Hitoshi Furusho

Fuyuki Sato

Keiko Kimura

Koji Wakui

Seiichi Nishizawa

Norio Teramae

Takehiro Seino

The present invention relates to a method for screening a nucleic acid aptamer comprising: (a) contacting a target molecule immobilized on a solid phase support with a nucleic acid aptamer candidate; (b) collecting the nucleic acid aptamer candidate binding with the target molecule by a capillary electrophoresis; and (c) amplifying the nucleic acid aptamer candidate by PCR.

Methods for screening nucleic acid aptamers

[Problem] To provide a novel nucleic acid aptamer for a vascular endothelial growth factor receptor, said nucleic acid aptamer being useful for the diagnosis and treatment of various diseases associated with VEGFs that can regulate angiogenesis and receptors for the VEGFs, e.g., tumor angiogenesis, diabetic retina and chronic rheumatoid arthritis. [Solution] A nucleic acid aptamer characterized by comprising a nucleotide sequence represented by any one of SEQ ID NOs: 1 to 5, and also characterized by being capable of bonding to a human VEGF receptor specifically. In a preferred embodiment of the nucleic acid aptamer, a primer recognition sequence, a fluorescent label, or a biotin molecule, an avidin molecule, a streptavidin molecule or other specific binding tag peptide may be linked to the 5'- or 3&#x2032;-terminal of the nucleic acid aptamer for the purpose of making it possible to detect the nucleic acid aptamer easily.

Nucleic acid aptamers binding to vascular endothelial growth factor receptors

Growing community of inventors

Tokyo, Japan

Keitaro Yoshimoto