Public University Corporation Yokohama City University

Ricoh Company, Ltd.

Corning Incorporated

Shibuya Corporation

Biomedica Solutions Co., Ltd.

Kuraray Co., Ltd.

Keisuke Sekine

Hideki Taniguchi

Takanori Takebe

Shinichiro Naruse

Soichi Saiga

Motohiro Fukuda

Go Tazaki

Tetsuya Nishimura

Masaya Hosoda

Atsushi Nakao

Yun-Wen Zheng

Yoko Itchoda

Hironobu Sunayama

Takashi Hiromatsu

Tomonori Naito

The present invention solves the following problems [1] to [3] found in conventional methods of preparing a three dimensional structure (organ primordium) by coculturing functional cells with umbilical cord-derived vascular endothelial cells and bone marrow-derived mesenchymal cells: [1] the quality of resultant organ primordia varies greatly depending on donors; [2] the growth capacities of cell sources are limited; and [3] it is difficult to secure immunocompatibility because cells are derived from different sources. An organ bud prepared from vascular cells, mesenchymal cells and tissue or organ cells, wherein each of the vascular cell, the mesenchymal cell and the tissue or organ cell has been induced from pluripotent stem cells. A method of preparing an organ bud, comprising culturing vascular cells, mesenchymal cells and tissue or organ cells in vitro, wherein each of the vascular cell, the mesenchymal cell and the tissue or organ cell has been induced from pluripotent stem cells.

Formation of three-dimensional organ from pluripotent stem cells

An operation isolator forms an aseptic space. An incubator is connected to the operation isolator, in which cells are stored and cultured. A storage chamber stores articles used in the operation isolator. In order to carry articles from the outside into the storage chamber, a decontamination pass-box is provided. The storage chamber and the operation isolator are directly or indirectly connected to each other.

Cell culture system

Provided is a method that achieves control of embryoid body size and can induce differentiation in a state where the embryoid body size is controlled, by using a cell culture chamber having a plurality of microchambers formed therein. A culture method for causing differentiation of pluripotent mammalian cells uses a cell culture chamber () having a plurality of microchambers () formed on a culture surface. The cell culture chamber () has a culture surface formed of spaces in which the microchambers () have a space structure with a height of 10 &#x3bc;m to 500 &#x3bc;m and a bottom area of 100 &#x3bc;mto 1 mm. The culture method for causing differentiation of pluripotent mammalian cells includes culturing pluripotent mammalian cells to obtain a cell population at least partially differentiated into endoderm lineage cells, by using the cell culture chambers ().

Method for differentiating pluripotent mammalian stem cells into a population of hepatic cells in a microchamber

An image forming apparatus includes a sheet feeder, a carriage, a cutter, a cutter unit, and a controller. A first standby position and a second standby position are disposed on both ends of a range of movement of the carriage. The carriage does not contact the cutter unit at each of the first standby position and the second standby position. The controller is configured to control the carriage and the cutter unit to overlappingly move. In an image recording condition in which images are consecutively recorded in a plurality of pages on a sheet, the controller is configured to change a direction of movement of the carriage at a leading end of a page so that the direction of movement of the carriage is the same as a direction of movement of the cutter unit at a sheet cutting position at which the cutter cuts the sheet.

Image forming apparatus

Growing community of inventors

Kanagawa, Japan

Keisuke Sekine