Showa Denko K.k.

Showa Denko Kabushiki Kaisha

Keisuke Mano

Eiji Ogata

Shinobu Ito

Tsutomu Nozawa

Kuniaki Shimbo

Yohei Kurata

Naoaki Misu

Takami Ooe

Yutaka Oonishi

A particulate hydrophobic polymer produced by reacting (A) a particulate crosslinked polymer having a hydroxyl group with (B) a crosslinkable epoxy compound, hydrolyzing the epoxy bonds, and then reacting the obtained compound having a hydroxyl group with (C) an epoxy compound having from 6 to 40 carbon atoms in total, a production process therefor, a column for reversed-phase liquid chromatography, and an analysis method using the column. By using the particulate hydrophobic polymer of the present invention having a remarkably high acid/alkali resistance, a sharp chromatogram for polycyclic aromatic compounds is obtainable with the swelling and shrinkage of the particulate polymer itself reduced and without reducing the column efficiency among various solvents even when the eluent in the column is variously exchanged.

Particulate hydrophobic polymer, production process therefor and column for reversed-phase high-performance liquid chromatography

The present invention relates to a packing agent for reversed phase liquid chromatography, including crosslinked polymer base particles which are obtained by aqueous suspension-polymerization of a crosslinkable vinyl ester or vinyl ether having a hydroxyl group within the molecule and which has an exclusion limit molecular weight of 100,000 or less in terms of standard polystyrene by size exclusion chromatography, wherein an acyl group having from 4 to 24 carbon atoms is ester-bonded to a part of hydroxyl groups in the crosslinked polymer base particles. The present invention also provides a production process of a packing agent, a column for reversed phase liquid chromatography using the packing agent, and an analysis method by reversed phase liquid chromatography using the packing agent.

Packing agent for reversed phase liquid chromatography and production process thereof

Disclosed herein are a highly purified tocopheryl phosphate and/or a salt thereof (tocopheryl phosphates) wherein a P,P'-bistocopheryl hypophosphate and/or a salt thereof (P,P'-bistocopheryl diphoshates) is contained in a proportion of not higher than 3% by weight; a process for producing a highly purified tocopheryl phosphate and/or a salt thereof, which comprises the steps of reacting a tocopherol with an oxyphosphorus trihalide followed by treating with an acid or basic aqueous solution to thereby form tocopheryl phosphates (i) in which P,P'-bistocopheryl diphoshates (ii) formed as by-products are contained, hydrolyzing the P,P'-bistocopheryl diphoshates (ii) under acid condition, and, optionally, rendering the hydrolyzate neutral or basic under basic condition; and a method of analyzing tocopheryl phosphates, comprising analyzing a sample containing components (i) and (ii) with the use of a high-performance liquid chromatograph column packed with a gel of a polymethacrylate having, bonded thereto, long-chain alkyl groups. None or only an extremely minute amount of P,P'-bistocopheryl diphoshates are contained in the highly purified tocopheryl phosphates, so that the highly purified tocopheryl phosphates exhibit antioxidant and blood circulation promoting effects, have excellent water solubility, are powdery so that the handling thereof is extremely easy, are free from cutaneous irritation and allergenecity and ensure dermal safety. Therefore, the highly purified tocopheryl phosphates are useful as cosmetic ingredients. The amounts of components (i) and (ii) can be simply measured with high accuracy by the above method.

Highly purified tocopheryl phosphate, process for producing the same,

High-  purity tocopherol phosphates, process for the preparation

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Kanagawa, Japan

Keisuke Mano