Toppan Printing Co., Ltd.

Elpida Memory, Inc.

Kabushiki Kaisha Meidensha

Michio Kawata, Director-General of Agency of Industrial Science and

Keisuke Goto

Yoichi Makino

Akihiro Hoshino

Yosuke Horiuchi

Tomoko Kunitomi

Sachiko Edo

Hideo Tsukune

An analysis apparatus including a stage, an analysis device placed on the stage and including receiving sections which accommodate a sample and a reagent for biochemical reaction, and are communicated with one another through a flow path having an inlet and an outlet, a liquid introduction section which is connected to the inlet and supplies into the flow path the sample, the reagent, and an sealing liquid for sealing each of the receiving sections, and a waste liquid storage section which is connected to the outlet and stores as waste liquid an excess of the sample and the reagent and a part of the sealing liquid supplied to the flow path, an optical system which includes an objective lens, emits excitation light to the receiving sections and allows observation of fluorescence generated in the receiving sections by the excitation light, and a control unit that controls such that the sealing liquid and the excess of the sample and the reagent form an interface in the waste liquid storage section, and that the interface is formed at a distance not less than a fluorescence-obtainable distance from a bottom of the receiving sections.

Analysis devices, analysis kits, and analysis systems

A microfluidic device includes a substrate having an electromagnetic wave transmission property, a lid member facing the substrate and being separated from the substrate such that a flow channel is formed between the substrate and the lid member, a light absorption layer which is placed in the flow channel and absorbs an electromagnetic wave, and a microwell array formed on the substrate and having plural microwells that are open to the flow channel to receive a target of analysis.

Microfluidic devices and observation methods

A microfluidic device includes: a base plate allowing an electromagnetic wave to pass therethrough and having no autofluorescence; a microwell array formed on the base plate and including a wall layer in which a plurality of through-holes are formed in a thickness direction; and a lid member disposed opposite to the base plate in a state of being separated from the wall layer, wherein microwells are formed by the base plate and the through-holes formed in the wall layer, and wherein the wall layer is formed of a material containing a colored component that absorbs an electromagnetic wave of a predetermined wavelength.

Microfluidic device and sample analysis method

A DLL circuit includes a delay circuit, a phase comparing circuit and a delay control circuit. The delay circuit is connected to first and second nodes, and delays an original clock signal supplied to the first node based on a delay control signal and generates first to n-th (n is an integer more than 1) internal clock signals. The first internal clock signal is outputted from the second node. Also, the internal clock signals other than the first internal clock signal are outputted from the delay circuit without passing through the second node, and lead the first internal clock signal in phase. The phase comparing circuit compares the original clock signal supplied from the first node and the first internal clock signal supplied from the second node, and outputs a phase difference of the original clock signal and the first internal clock signal. The delay control circuit outputs the delay control signal to the delay circuit based on the phase difference outputted from the phase comparing circuit.

DLL circuit and method of generating timing signals

In order to define the contour of an ellipse, it is necessary to determine five unknown parameters in the general equation representative of centered conics. When directly applying Hough transformation method to the above equation, since a five-dimensional space is required, it is practically impossible to detect an ellipse because a long processing time and a great amount of memory capacity are inevitably required. To overcome these problems, the geometric properties of an ellipse are determined separately on three parameter sub-spaces obtained on the basis of edge vector field: two-dimensional center histogram and two-dimensional (H, B) histogram, one-dimensional C histogram. A peak value on the center histogram represents a group of ellipse having the same center locations; a peak value on the (H, B) histogram represents a group of concentric ellipse having the same eccentricity and axis slope; a peak value on the C histogram defines a single ellipse. By sequentially selecting these peaks in the three sub-spaces, it is possible to define geometric properties of an ellipse under practical processing conditions. In order to define a plurality of ellipses efficiently, several novel methods have been adopted: separation of the rotation directions of edge vector field, recovery of edge vectors having no mate edge vectors; adoption of least mean square method, etc., in particular.

Growing community of inventors

Tokyo, Japan

Keisuke Goto