Kyowa Hakko Kogyo Co., Ltd.

Kyowa Hakko Bio Co., Ltd.

Kyowa Hakko Food Specialties Co., Ltd.

Keiko Ochiai

Katsuhiko Ando

Akio Ozaki

Hideo Mori

Tamio Mizukami

Shigeru Chiba

Yoichi Uosaki

Takeshi Shibasaki

Shiro Akinaga

Chiaki Saitoh

Masato Ikeda

Ryoichi Katsumata

Kazuo Aisaka

Youichi Uosaki

Haruhiko Yokoi

Junko Ohnishi

Yoshinori Yamashita

Hirofumi Nakano

Akira Asai

Isao Kawamoto

Satoshi Mitsuhashi

Yoshiyuki Yonetani

Yasushi Sakai

Yukio Hashimoto

Toru Mizukami

Hiroaki Motoyama

Tsutomu Agatsuma

Hideki Kawasaki

Akira Mihara

Yutaka Takano

Masaru Hasegawa

Nobuo Ogata

Tetsuo Yoshida

Atsushi Yashiro

Yumiko Aotani

Seiko Ando

Keishiro Tamura

Hideyo Kumazawa

Hiroshi Katahira

Atsuko Yokoi

Kyoko Ozawa

Chikara Tokunaga

Shinichi Hashimoto

Yutaka Saitoh

Satoshi Nakagawa

Tadashi Ashizawa

Eiji Tsukuda

Shin-ichi Hashimoto

Tatsuya Tamaoki

Yuzuru Matsuda

Isami Takahashi

Mayumi Yoshida

Mitsunobu Hara

Mikiro Hayashi

Takeo Tanaka

Kozo Ouchi

Hidemasa Kondo

Hiroshi Mizoguchi

Hiroyuki Nagata

Shingo Kakita

Sakae Tazoe

Fumito Koizumi

Atsuhiro Hasegawa

Ritsuko Katahira

Mikito Itoh

Akihiro Senoh

Naoko Tateishi

The present invention relates to a polypeptide having a modified amino acid sequence of 6-phosphogluconate dehydrogenase (hereinafter abbreviated as GND) derived from a microorganism belonging to the genus, said modification being substitution of the amino acid residue(s) at the position(s) corresponding to the 158th and/or the 361st amino acid(s) of the amino acid sequence shown in SEQ ID NO: 1, and having GND activity; DNA encoding the polypeptide; a recombinant DNA comprising the DNA; a transformant carrying the recombinant DNA; a microorganism carrying the DNA on the chromosome; and a process for producing a useful substance which comprises culturing the transformant or the microorganism in a medium.

Mutant 6-phosphogluconate dehydrogenase

Mutated 6-phosphogluconate dehydrogenase

Novel polynucleotides derived from microorganisms belonging to coryneform bacteria and fragments thereof, polypeptides encoded by the polynucleotides and fragments thereof, polynucleotide arrays comprising the polynucleotides and fragments thereof, recording media in which the nucleotide sequences of the polynucleotide and fragments thereof have been recorded which are readable in a computer, and use of them.

[object Object]

An isolated gene encodes for a L-proline-3-hydroxylase having the amino acid sequence of SEQ ID NOS: 1, 2, 15, 16 and 17, or for a protein having enzymatic activity to hydroxylate the 3-position of L-proline and to act on free L-proline in the presence of 2-ketoglutaric acid and divalent iron ions to produce cis-3-hydroxy-L-proline. The gene is inserted into a vector and the resulting recombinant DNA is used to create a transformant. L-proline-3-hydroxylase is produced by culturing the transformant in a medium.

Process for producing cis-3-hydroxy-L-proline

The present invention relates to a novel glucose-6-phosphate dehydrogenase (hereinafter referred to as 'G6PD') derived from a bacterium belonging to the genus, a DNA encoding the enzyme, a recombinant DNA comprising the DNA, a transformant comprising the recombinant DNA, a transformant comprising the DNA on its chromosome, and a process for producing L-amino acid or G6PD which comprises culturing the transformant. According to the present invention, a modified G6PD and a DNA encoding the G6PD are obtained, and the productivity of L-amino acid by a microorganism can be improved by using the modified G6PD.

Glucose-6-phosphate dehydrogenase

The present invention relates to a novel flavoring solution for making pickles having a flavor of foods pickled in rice bran paste (hereinafter referred to as a bran pickles flavoring solution), a clear bran pickles flavoring solution, processes for producing said solutions, a novel microorganism belonging to the genusused for the production of said flavoring solutions, and a process for producing a solution containing a lactone wherein the lactone is selectively extracted from a culture of a lactone-producing microorganism. The present invention also relates to a novel bacterium of the genuswhich has the ability to produce &#x3b3;-dodecalactone and/or &#x3b3;-dodecelactone and is useful for the production of said bran pickles flavoring solutions.

Process for producing a bran pickles flavoring solution

The present invention relates to a novel aspartokinase derived from a Coryneform bacterium; a DNA encoding the enzyme; a recombinant DNA containing the above DNA; a Coryneform bacterium having the above recombinant DNA or a Coryneform bacterium having the DNA on its chromosome; and a process for producing L-lysine by culturing the above microorganism. Construction has been successfully made of a DNA encoding an aspartokinase freed from concerted feedback inhibition by L-lysine and L-threonine derived from aand has a nucleotide sequence encoding an amino acid sequence wherein the amino acid residue at position 311 is an amino acid other than Thr in the amino acid sequence shown by SEQ ID NO: 18.

Desensitized aspartokinase

CHOLESTEROL DEHYDROGENASE, COPROSTAN-3-ONE DEHYDROGENASE AND 4-CHOLESTEN-3-ONE DEHYDROGENASE, COMPOSITIONS CONTAINING THE DEHYDROGENASES, AND METHOD FOR REDUCING AMOUNT OF CHOLESTEROL USING THE COMPOSITIONS

Bacillus strains which produce HMG-CoA reductase inhibitors

An industrially applicable process for producing cis-3-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process, L-proline is converted into cis-3-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genus Streptomyces or Bacillus and which catalyzes hydroxylation of L-proline into cis-3-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced cis-3-hydroxy-L-proline is collected from the aqueous medium. A novel enzyme L-proline-3-hydroxylase, a gene of L-proline-3-hydroxylase which is useful for the process, a transformant containing the gene, and a process for producing L-proline-3-hydroxylase using the transformant.

Process for producing CIS-3-hydroxy-L-proline

A flavoring solution for making pickles having a flavor of foods pickled in rice bran paste, a clear bran pickles flavoring solution, processes for producing the flavoring solutions, a process for producing lactones which are essential components of the flavoring solutions, and a bacterium belonging to the genus Corynebacterium which is useful for producing a lactone are disclosed. The bacterium is preferably Corynebacterium sp. NK-1 (FERM BP-6329). The lactone is preferably a &ggr; lactone.

Process for producing a lactone using corynebacterium sp. NK-1 (FERM BP-6329)

An enzymatic process is presented for reducing the cholesterol level of foods and feeds by converting the cholesterol therein to coprostanol which has a very low intestinal tract absorbability. Cholesterol in meat, egg, milk, seafood and cooked processed foods containing the same, or feeds for animals, poultry, and pisiculture can be reduced by the sequential action of three enzymes isolated from a eubacterium: cholesterol dehydrogenase, 4-cholesten-3-one dehydrogenase and coprostan-3-one dehydrogenase.

Process for producing a cholesterol-reduced substance

Disclosed is a process for producing a compound represented by general formula (II-a):

Process for the preparation of HMG-COA reductase inhibitors

Method for quantitative determination of 1,5-anhydroglucitol

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Ebina, Japan

Keiko Ochiai