Riken Corporation

Sumitomo Chemical Company, Limited

Sumitomo Bakelite Company Limited

Keiko Muguruma

Yoshiki Sasai

Hayao Tanaka

Ryouhei Tsukada

Yoshiki (deceased) Sasai

The present invention provides a method for producing a human cell aggregate containing a midbrain-hindbrain boundary neural progenitor tissue, including subjecting an aggregate of human pluripotent stem cells to suspension culturing in a serum-free medium containing insulin, and treating, in the suspension culturing, the aggregate of human pluripotent stem cells or a human cell aggregate derived therefrom with a ROCK inhibitor, a TGF&#x3b2; signal inhibitor, and a first fibroblast growth factor. Furthermore, the human cell aggregate containing the midbrain-hindbrain boundary neural progenitor tissue is subjected to suspension culturing in a serum-free medium to induce formation of a neuroepithelial structure by neural progenitor in the neural progenitor tissue, whereby the human cell aggregate containing the cerebellar plate tissue can be obtained.

Method for producing cerebellar progenitor tissue

Provided are a culture vessel with which an embryonic body can be formed efficiently from human embryonic stem cells, and a method for culturing human embryonic stem cells using the vessel. There is provided a vessel for culturing human embryonic stem cells, the culture vessel having two or more wells (), wherein each of the wells () has a tubular body () and a funnel-shaped bottom () provided at one end of the body (), the bottom () being a concave curved surface at the center () of the bottom () and the bottom () having an opening angle (&#x3b8;) in range of 60 to 100&#xb0;. There is provided a method for culturing human embryonic stem cells by using said vessel for culturing human embryonic stem cells.

Vessel for culturing human ES cells

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Wako, Japan

Keiko Muguruma