Kyushu University

Keiko Kobayashi

Takahiro Nakamura

Yusuke Yagi

A method for designing a protein capable of binding in an RNA base selective manner or RNA base sequence specific manner is provided. The protein of the present invention is a protein containing one or more of PPR motifs (preferably 2 to 14 PPR motifs) each consisting of a polypeptide of 30- to 38-amino acid length represented by the formula 1 (wherein Helix A is a moiety of 12-amino acid length capable of forming an &#x3b1;-helix structure, and is represented by the formula 2, wherein, in the formula 2, Ato Aindependently represent an amino acid; X does not exist, or is a moiety of 1- to 9-amino acid length; Helix B is a moiety of 11- to 13-amino acid length capable of forming an &#x3b1;-helix structure; and L is a moiety of 2- to 7-amino acid length represented by the formula 3, wherein, in the formula 3, the amino acids are numbered 'i' (&#x2212;1), 'ii' (&#x2212;2), and so on from the C-terminus side, provided that Lto Lmay not exist), and combination of three amino acids A, Aand L, or combination of two amino acids A, and Lis a combination corresponding to a target RNA base or base sequence.

Method for designing RNA-binding protein utilizing PPR motif, and use thereof

A method for designing a protein capable of binding in an RNA base selective manner or RNA base sequence specific manner is provided. The protein of the present invention is a protein containing one or more of PPR motifs (preferably 2 to 14 PPR motifs) each consisting of a polypeptide of 30- to 38-amino acid length represented by the formula 1 (wherein Helix A is a moiety of 12-amino acid length capable of forming an &#x3b1;-helix structure, and is represented by the formula 2, wherein, in the formula 2, Ato Aindependently represent an amino acid; X does not exist, or is a moiety of 1- to 9-amino acid length; Helix B is a moiety of 11- to 13-amino acid length capable of forming an &#x3b1;-helix structure; and L is a moiety of 2- to 7-amino acid length represented by the formula 3, wherein, in the formula 3, the amino acids are numbered 'i' (&#x2212;1), 'ii' (&#x2212;2), and so on from the C-terminus side, provided that Lto Lmay not exist), and combination of three amino acids A, Aand Lor combination of two amino acids A, and Lis a combination corresponding to a target RNA base or base sequence.

Method for designing RNA binding protein utilizing PPR motif, and use thereof

A method for designing a protein capable of binding in an RNA base selective manner or RNA base sequence specific manner is provided. The protein of the present invention is a protein containing one or more of PPR motifs (preferably 2 to 14 PPR motifs) each consisting of a polypeptide of 30- to 38-amino acid length represented by the formula 1 (wherein Helix A is a moiety of 12-amino acid length capable of forming an &#x3b1;-helix structure, and is represented by the formula 2, wherein, in the formula 2, Ato Aindependently represent an amino acid; X does not exist, or is a moiety of 1- to 9-amino acid length; Helix B is a moiety of 11- to 13-amino acid length capable of forming an &#x3b1;-helix structure; and L is a moiety of 2- to 7-amino acid length represented by the formula 3, wherein, in the formula 3, the amino acids are numbered 'i' (-1), 'ii' (-2), and so on from the C-terminus side, provided that Lto Lmay not exist), and combination of three amino acids A, Aand L, or combination of two amino acids A, and Lis a combination corresponding to a target RNA base or base sequence.

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Fukuoka, Japan

Keiko Kobayashi