Kabushiki Kaisha Toshiba

Sekisui Medical Co., Ltd.

Keiko Ito

Koji Hashimoto

Mika Inada

Naoko Nakamura

Nobuhiro Gemma

Yoshio Ishimori

Osamu Sato

Masayoshi Takahashi

Michie Hashimoto

Hideki Horiuchi

Masanori Gotoh

According to one embodiment, a method of quantifying a target nucleic acid containing a first sequence in a sample is provided. The method includes preparing a substrate on which a plurality of detection regions are arranged, forming a reaction field by placing, on the substrate, a reaction liquid containing a sample, a primer set, and an amplification enzyme, maintaining the reaction field in an isothermal amplification condition, detecting a detection signal for each of the detection regions, determining, for each of the plurality of detection regions, whether positive or negative and detecting or quantifying the target nucleic acid based on the number of positive and/or a rise time of each of the positive detection signal.

Nucleic acid detection or quantification method, chip and assay kit therefor, device for detecting or quantifying nucleic acid and program therefor

According to one embodiment, a primer set for elongating a target short-chain nucleic acid containing a first sequence to obtain an elongated product is provided. The elongated product contains a second, a third, a fourth sequence, a complementary sequence of the 1'-th sequence and a sixth sequence. The complementary sequence of the 1&#x2032;-th sequence is a loop primer sequence. The primer set contains a first elongation primer containing a first elongation primer sequence and a complementary sequence of the sixth sequence, and a second elongation primer containing a second elongation primer sequence, the fourth, the third, and the second sequence.

Short-chain nucleic acid elongation primer set, assay kit, and short-chain nucleic acid elongation, amplification and detection methods

According to one embodiment, a nucleic acid reaction tool includes a support having a first surface, a covering body having a second surface, and a groove opened on the second surface, and a primer set. The covering body is in contact with the support to form a reaction space surrounded by the first surface and the groove. The groove includes, on an inner surface of the reaction space, rising surfaces opposed to each other, and a rear surface connecting one end of the side surfaces, and a primer fixing region to which the primer set is fixed, the primer fixing region being located at a corner where the one end of the side surfaces connected to the rear surface in the reaction space.

Nucleic acid reaction tool, nucleic acid detection/quantification kit, and nucleic acid detection/quantification method

According to one embodiment, a detection method is a method for detecting a plurality of target nucleic acids in a sample. The method includes (a) preparing a chain-elongation nucleic acid set group, a primer set, and a probe immobilized substrate, (b) obtaining the target nucleic acid and a long-chain nucleic acid group containing a first sub-chain-elongation nucleic acid and a second sub-chain-elongation nucleic acid, (c) obtaining an amplification product group by maintaining the long-chain nucleic acid group and the primer set under amplification conditions, (d) detecting presence/absence and/or an amount of hybridization, and (e) detecting the plurality of target nucleic acids.

Method for detecting a plurality of short-chain nucleic acid in sample, combinatorial analysis kit, analysis kit supply management method

In general, according to one embodiment, a method containing, reverse-transcribing the first sequence in the target RNA, to obtain a reverse-transcription product containing a first' sequence complementary to the first sequence, dissociating the reverse-transcription product from the target RNA, hybridizing an elongation primer and the reverse-transcription product to elongate both, thereby obtain an elongation product, and maintaining the amplification reaction liquid containing the elongation product, a primer set and Tin(exo-) DNA polymerase and/or Bsm DNA polymerase under an amplification reaction condition, to amplify the first&#x2032; sequence.

RNA amplification method, RNA detection method and assay kit

The nucleic acid detection method includes maintaining a reaction liquid containing the sample, a marker substance, polymerase, a primer set, and salt of a predetermined concentration in the reaction field which include a substrate and a probe, under an amplification condition, detecting a signal from the marker substance and determining existence and/or quantity of the target nucleic acid. The predetermined concentration is higher than the concentration in which the peak rate of the amplification reaction and the concentration with which the hybridization rate becomes higher than 0 au, whichever is higher, but not greater than the highest one of the concentrations with which the quantity of detection of the amplification product becomes a threshold value or more.

Nucleic acid detection method

According to one embodiment, a method for detecting target nucleic acid includes the following steps. (A) A reaction field is formed by placing a reaction mixture on an electrode, and the reaction mixture contains the sample, a primer set, an amplification enzyme, 4 mM to 30 mM of magnesium ion, and a redox probe. The redox probe has an oxidation reduction potential, which generates an electric signal of which amplitude increases. (B) The reaction field is maintained under an amplification reaction condition. (C) The electric signal is detected with the electrode. (D) Existence or quantity of the target nucleic acid is determined.

Nucleic acid detection method and assay kit

Kits for use in a method of detecting an amplification product by hybridizing it with a probe, the amplification product is amplified from a target nucleic acid with the primers, including placing F3, F2 and F1 regions in this order from a 5' terminal side and B3, B2and B1regions in this order from a 3&#x2032; terminal side, and additionally an FP region in the region from the F2 to F1 regions and/or a BPc region in the region from the B2to B1regions in the target nucleic acid, determining the respective regions in such a manner that the FP and F2 regions and/or the BPc and B2regions have an unoverlapping region of at least 10 bases or more and overlapping regions of 10 bases or less, and designing the primers according to the regions.

Assay kit for use in method of detecting a target nucleic acid

There is provided a nucleotide primer set for LAMP amplification, used for detecting genotypes of single-nucleotide polymorphisms G590A, G857A and T341C of a NAT2 gene. There is also provided a nucleotide probe for detection of an amplification product amplified with the primer set according to the present invention. There is also provided a method of detecting the genotypes of NAT2 gene single-nucleotide polymorphisms G590A, G857A and T341C by using the primer set according to the present invention.

Nucleotide primer set and nucleotide probe for detecting genotype of N-acetyltransferase-2 (NAT2)

Provided is a nucleic acid primer for LAMP amplification for use in the detection of human papilloma virus and identification of its genotype. The present invention also provides a method of detecting human papilloma virus and identifying its genotype, includes a step of amplifying the nucleic acid chains in a sample in LAMP reaction by using multiple primers including at least one primer selected from the nucleic acid primers according to the present invention and a step of detecting presence of amplified products after the amplification reaction and identifying their genotypes.

Method of detecting human papilloma virus by using nucleic acid amplification method and nucleic acid chain-immobilized carrier

A method of designing primers for use in a method of detecting an amplification product by hybridizing it with a probe, the amplification product is amplified from a target nucleic acid with the primers, including placing F3, F2 and F1 regions in this order from a 5' terminal side and Bc, B2and B1regions in this order from a 3&#x2032; terminal side, and additionally an FP region in the region from the F2 to F1 regions and/or a BPc region in the region from the B2to B1regions in the target nucleic acid, determining the respective regions in such a manner that the FP and F2 regions and/or the BPc and B2regions have an unoverlapping region of at least 10 bases or more and overlapping regions of 10 bases or less, and designing the primers according to the regions.

Method of detecting amplification product or target nucleic acid

There is provided is a nucleotide primer set for LAMP amplification used for detecting genotypes of single-nucleotide polymorphisms C677T and A1298C of an MTHFR gene. There is also provided a nucleotide probe for detecting an amplification product amplified by the primer set according to the present invention. There is also provided a method of detecting the genotypes of the single-nucleotide polymorphisms C677T and A1298C in the MTHFR gene, by using the primer set according to the present invention.

Nucleotide primer set and nucleotide probe for detecting genotype of methylene tetrahydrofolate reductase (MTHFR)

A single stranded nucleic acid probe having a base sequence complementary to the gene to be detected is immobilized onto the surface of an electrode or the tip of an optical fiber, and the nucleic probe is reacted with the gene sample denatured to a single stranded form, and then the nucleic acid probe hybridized with the gene is detected. In this procedure, to the reaction system consisting of the nucleic acid probe and the gene sample, a double stranded nucleic acid recognizing substance capable of binding specifically to the double stranded nucleic acid and being active electrochemically or optically is added. The detection of the nucleic acid probe is conducted by electrochemical or optical determination utilizing the electrode or optical fiber mentioned above. By this method, safer and more convenient detection of the gene is possible at a higher sensitivity even in a reduced time period.

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Kawasaki, Japan

Keiko Ito