Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo

Hayashibara Co., Ltd.

Kagawa University

Matsutani Chemical Industry Co., Ltd.

Rare Sugar Production Technical Research Laboratories, Llc.

Teikoku Seiyaku Co., Ltd.

Fushimi Pharmaceutical Company, Limited

National University Corporation Kagawa Univesity

Keiji Tsusaki

Michio Kubota

Ken Izumori

Toshiyuki Sugimoto

Shigeharu Fukuda

Hiroto Chaen

Toshio Miyake

Tomoyuki Nishimoto

Hikaru Watanabe

Masaaki Tokuda

Kazuko Hattori

Kazuhiko Maruta

Kazuyuki Oku

Hikaru Masahiro Watanabe

Takuo Yamamoto

Kazuhiro Okuma

Takanobu Higashiyama

Yoshio Tsujisaka

Kei Takeshita

Mitsuhiro Nagata

Goro Takata

Kenji Morimoto

Koji Murao

Masaaki Ueki

Yutaka Ishida

Mohammad Akram Hossain

Toshifumi Itano

Osamu Miyamoto

Toshihiko Ishida

Taizo Tasaka

Gan Muneuchi

Fuminori Yamaguchi

Terukazu Tanaka

Kazuyuki Hirooka

Tomohiko Taminato

Takeo Takahashi

Kazuhisa Mukai

George Fleet

Mohammed Akram Hossain

Asahiko Taminato

The present invention has objects to provide a glucan useful as water-soluble dietary fiber, its preparation and uses. The present invention solves the above objects by providing a branched &#x3b1;-glucan, which is constructed by glucose molecules and characterized by methylation analysis as follows:

Branched alpha-glucan, alpha-glucosyltransferase which forms the glucan, their preparation and uses

Branched &#x3b1;-glucan, &#x3b1;-glucosyltransferase which forms the glucan, their preparation and uses

The object of the present invention is to provide a method and a process for producing 2-O-&#x3b1;-glucopyranosyl-L-ascorbic acid where 5-O-&#x3b1;-glucopyranosyl-L-ascorbic acid and 6-O-&#x3b1;-glucopyranosyl-L-ascorbic acid are not formed or formed in such a small amount that the formation of these can nor be detected. The present invention solves the above object by providing a process for producing 2-O-&#x3b1;-glucopyranosyl-L-ascorbic acid comprising the steps of allowing &#x3b1;-isomaltosyl glucosaccharide-forming enzyme together with or without cyclomaltodextrin glucanotransferase (EC 2.4.1.19) to act on a solution comprising L-ascorbic acid and, an &#x3b1;-glucosyl saccharide to form 2-O-&#x3b1;-glucopyranosyl-L-ascorbic acid and collecting the formed 2-O-&#x3b1;-glucopyranosyl-L-ascorbic acid.

Process for producing 2-O-alpha-D-glucopyranosyl-L-ascorbic acid

Problem: To provide a microorganism with an ability to produce deoxy polyol dehydrogenase. Means for Resolution: A microorganism belonging to genuswith an ability to produce a dehydrogenase for deoxy polyol of the same structure at the positions C2 and C3 as that of ribitol or L-iditol. The bacterial cell IK7 of the genus(accession No. NITE P-271). A method for producing deoxy ketose comprising allowing a culture containing the deoxy polyol dehydrogenase obtained by the culturing of the microorganism of the invention or allowing the deoxy polyol dehydrogenase to react with a solution containing deoxy polyol of the same structure at the positions C2 and C3 as that of ribitol or L-iditol to oxidize deoxy polyol to produce the corresponding deoxy ketose and then collecting the deoxy ketose. The deoxy polyol is 1-deoxy-D-allitol, while the corresponding deoxy ketose is 1-deoxy-L-psicose. Otherwise, the deoxy polyol is L-rhamnitol, while the corresponding deoxy ketose is 1-deoxy-L-fructose.

Microorganism with ability to produce deoxy polyol dehydrogenase and use thereof

A method of utilizing the physiological activity of a rare saccharide, wherein physiological-activity sensitive cells are treated with the rare saccharide to modify the function of the cells. A composition containing, as an active ingredient, a rare saccharide which is introduced into physiological-activity sensitive cells and has an effect of modifying the function of the cells. The cells are human cells. The composition is a functional food, a drug, or a cosmetic. The rare saccharide is a rare saccharide belonging to aldose and/or ketose. The aldose is D-allose, and the cells are selected from the group consisting of cancer-cell proliferation inhibitory activity sensitive cells and active-oxygen production inhibitory activity sensitive cells. The ketose is D-psicose, and the cells are selected from the group consisting of chemokine secretion inhibitory activity sensitive cells, microglia migration inhibitory activity sensitive cells, and hypoglycemic activity sensitive cells.

Method of utilizing physiological activity of rare saccharide and composition containing rare saccharide

Providing 1- or 6-deoxy products corresponding to all of aldohexoses, ketohexoses and sugar alcohols, as based on Deoxy-Izumoring, as well as a method for systematically producing those products. A method for producing deoxyketohexose and a derivative thereof using a deoxyketohexose isomerase derived fromST-24 (FERM BP-2736), comprising epimerizing 1-deoxy D-ketohexose or 6-deoxy D-ketohexose or 1-deoxy L-ketohexose or 6-deoxy L-ketohexose at position 3 to produce the individually corresponding 1-deoxy D-ketohexose or 6-deoxy D-ketohexose or 1-deoxy L-ketohexose or 6-deoxy L-ketohexose as an intended product.

Deoxyketohexose isomerase and method for producing deoxyhexose and derivative thereof using same

Object: To provide a thermostable L-ribose isomerase. Means for Resolution: The thermostable L-ribose isomerase with MW. 32,000 (by SDS-PAGE), optimal temperature of 45&#xb0; C., optimal pH of pH 9.0 (glycine-NaOH buffer), and stable physicochemical properties such as temperature stability up to 45&#xb0; C. during thermal treatment at pH 9.0 for 10 minutes, and with an action to isomerize L-ribose to generate L-ribulose or of inversely to isomerize L-ribulose to generate L-ribose. A conversion method between an aldose and a ketose comprising allowing the thermostable L-ribose isomerase as an enzyme derived from (1)strain MB426 (NITE BP-277) to interact with an aldose selected from L-ribose, D-lyxose, D-tallose, D-mannose, L-allose and L-gulose to isomerize the aldose to generate a ketose selected from the individually corresponding L-ribulose, D-xylulose, D-tagatose, D-fructose, L-psicose and L-sorbose or to interact with a ketose selected from L-ribulose, D-xylulose, D-tagatose, D-fructose, L-psicose and L-sorbose to isomerize the ketose to generate an aldose selected from the individually corresponding L-ribose, D-lyxose, D-tallose, D-mannose, L-allose and L-gulose.

Thermostable L-ribose isomerase and method for producing same and use of same

The object of the present invention is to provide an &#x3b1;-isomaltosylglucosaccharide-forming enzyme, process of the same, cyclotetrasaccharide, and saccharide composition comprising the saccharide which are obtainable by using the enzyme; and is solved by establishing an &#x3b1;-isomaltosylglucosaccharide-forming enzyme which forms a saccharide, having a glucose polymerization degree of at least three and having both the &#x3b1;-1,6 glucosidic linkage as a linkage at the non-reducing end and the &#x3b1;-1,4 glucosidic linkage other than the linkage at the non-reducing end, by catalyzing the &#x3b1;-glucosyl-transfer from a saccharide having a glucose polymerization degree of at least two and having the &#x3b1;-1,4 glucosidic linkage as a linkage at the non-reducing end without substantially increasing the reducing power; &#x3b1;-isomaltosyl-transferring method using the enzyme; method for forming &#x3b1;-isomaltosylglucosaccharide; process for producing a cyclotetrasaccharide having the structure of cyclo{66)-&#x3b1;-D-glucopyranosyl-(163)-&#x3b1;-D-glucopyranosyl-(166)-&#x3b1;-D-glucopyranosyl-(163)-&#x3b1;-D-glucopyranosyl-(16} using both the &#x3b1;-isomaltosylglucosaccharide-forming enzyme and the &#x3b1;-isomaltosyl-transferring enzyme; and the uses of the saccharides obtainable therewith.

&#x3b1;-isomaltosylglucosaccharide-forming enzyme, process and uses of the same

The object of the present invention is to provide an &#x3b1;-isomaltosylglucosaccharide-forming enzyme, process of the same, cyclotetrasaccharide, and saccharide composition comprising the saccharide which are obtainable by using the enzyme; and is solved by establishing an &#x3b1;-isomaltosylglucosaccharide-forming enzyme which forms a saccharide, having a glucose polymerization degree of at least three and having both the &#x3b1;-1,6 glucosidic linkage as a linkage at the non-reducing end and the &#x3b1;-1,4 glucosidic linkage other than the linkage at the non-reducing end, by catalyzing the &#x3b1;-glucosyl-transfer from a saccharide having a glucose polymerization degree of at least two and having the &#x3b1;-1,4 glucosidic linkage as a linkage at the non-reducing end without substantially increasing the reducing power; &#x3b1;-isomaltosyl-transferring method using the enzyme; method for forming &#x3b1;-isomaltosylglucosaccharide; process for producing a cyclotetrasaccharide having the structure of cyclo{&#x2192;6)-&#x3b1;-D-glucopyranosyl-(1&#x2192;3)-&#x3b1;-D-glucopyranosyl-(1&#x2192;6)-&#x3b1;-D-glucopyranosyl-(1&#x2192;3)-&#x3b1;-D-glucopyranosyl-(1&#x2192;} using both the &#x3b1;-isomaltosylglucosaccharide-forming enzyme and the &#x3b1;-isomaltosyl-transferring enzyme; and the uses of the saccharides obtainable therewith.

&#x3b1;-Isomaltosylglucosaccharide synthase, process for producing the same and use thereof

A polypeptide having &bgr;-fructofuranosidase activity is obtained by the expression of a microbial gene, which polypeptide contains the amino acid sequences of SEQ ID NO:1 and SEQ ID NO:2 in whole or in part as an amino acid sequence. This polypeptide is useful for producing fructofuranosyl transferred saccharides.

Polypeptide having &bgr;-fructofuranosidase activity

Disclosed are a polypeptide having a &bgr;-fructofuranosidase activity obtainable by the expression of a microbial gene, a DNA encoding the polypeptide, a transformant obtained by introducing the DNA into an appropriate host, a process for producing the polypeptide comprising culturing the transformant to produce the polypeptide and collecting the produced polypeptide from the culture, and a method for fructofuranosyl transfer comprising a step of reacting a fructofuranosyl donor with a fructofuranosyl acceptor in the presence of the polypeptide.

Polypeptides having &bgr;-fructofuranosidase activity

An L-ribose isomerase which isomerizes aldoses such as L-ribose, D-lyxose, D-talose, D-mannose, L-allose and L-gulose into their corresponding ketoses such as L-ribulose, D-xylulose, D-tagatose, D-fructose, L-psicose and L-sorbose. The enzymatic reaction is a reversible equilibrium reaction. The L-ribose isomerase can be obtained from microorganisms of the genus Acinetobacter.

L-ribose isomerase, its preparation and uses

Growing community of inventors

Okayama, Japan

Keiji Tsusaki