Riken Corporation

Spiber Inc.

Keiji Numata

Takeshi Yoshizumi

Yutaka Kodama

Junichi Sugahara

Taku Demura

Takashi Yamamoto

Yoshiharu Doi

Tetsushi Sakuma

Keisuke Morita

Hiroshi Ezura

Yunosuke Abe

Misato Ohtani

Takehisa Maekawa

Koichi Kotaka

Takaaki Miyamoto

Yu Miyagi

Kosuke Tsuchiya

Miwa Yamada

Hongfang Chi

Takaoki Ishii

Kousuke Tsuchiya

Kenji Miura

Yoko Horii

The present invention relates to a modified fibroin including a domain sequence represented by Formula 1: [(A)motif-REP]or Formula 2: [(A)motif-REP]&#x2212;(A)motif, in which the domain sequence has an amino acid sequence with a reduced content of a glutamine residue equivalent to an amino acid in which one or a plurality of glutamine residues in REP are deleted or substituted with other amino acid residues, as compared with a naturally occurring fibroin.

Modified fibroin

The present application provides: a polypeptide characterized by having an ability of penetrating into a cell and the structure represented by Formula (I) wherein n is a number of 2 to 20; Rand Rmay independently represent a C-Calkyl group or may bind to each other to form a ring; Rrepresents a C-Cprimary aminoalkyl group; Rrepresents a hydrogen atom or a C-Chydrocarbon group; the 'n' number of R, R, R, and Rmay independently be the same with or different from each other; and the N-terminus and the C-terminus may or may not be modified independently; and a cell-invasive composition comprising the polypeptide and a substance of interest.

Polypeptide comprising cell-penetrating sequence and composition comprising same

It is an object of the present invention to provide a method for introducing a protein into a plant, which is simple and extensively applicable to various types of plant cells and proteins. The above object is achieved by the present invention to provide a complex comprising a protein of interest to be introduced into a target plant cell and a carrier peptide, a method for introducing a protein of interest into a target plant cell using the complex, and a kit comprising a protein of interest to be introduced into a target plant cell and a carrier peptide.

Method for introducing protein into plant cell

Provided are a composite molding composition such as a composite film and a composite fiber that have properties such as an improved tensile strength, strain and toughness as a result of blending a peptide or polyamino acid having a &#x3b2;-sheet structure with a natural or artificially modified fibroin-derived protein; a method for producing such composite molding composition; and a method for improving the physical properties of a composite molding composition containing a fibroin-derived protein.

Composite molding composition including fibroin-like protein, and method for producing composite molding composition

The object of the present invention is to provide a method of introducing a nucleic acid into plant cells, which is simple and widely applicable to various types of plant cells and nucleic acids. The present invention relates to a method of introducing a nucleic acid into a target plant cell, comprising a step of forming a complex by bringing a carrier peptide, wherein the carrier peptide comprises a cell-penetrating sequence and a polycationic sequence, into contact with a nucleic acid and a step of bringing the obtained complex into contact with a target plant cell.

Method of introducing nucleic acid into plant cells

An object of the present invention is to provide a plant transformation method that is convenient and is widely applicable to various types of plant cells and nucleic acids. The present invention relates to a method for transforming a target plant, comprising the steps of: a) contacting a carrier peptide comprising a cell-penetrating sequence and a polycation sequence with a nucleic acid to form a complex; b) contacting the obtained complex with a cell of a meristem of the target plant to transfer the nucleic acid to the genome; c) allowing the meristem to grow; and d) selecting a plant harboring the transferred nucleic acid.

Plant transformation method

A polyhydroxyalkanoate copolymer of 3HB (3-hydroxybutyrate), 3HP (3-hydroxypropionate) and 5HV (5 -hydroxyvalerate) disclosed. The polyhydroxyalkanoate can be used for bioplastic or biomaterial. A method for producing the polyhydroxyalkanoate comprises culturing a microorganism belonging togenus in a culture medium that comprises lactone and/or hydroxy acid or salt of hydroxy acid as a carbon source.

Method for producing polyhydroxyalkanoate having long main chain structure

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Saitama, Japan

Keiji Numata