Kirin Beer Kabushiki Kaisha

Marine Biotechnology Institute Co., Ltd.

Kajima Corporation

Keiji Kondo

Norihiko Misawa

Susumu Kajiwara

Akihiro Yokoyama

Yutaka Miura

Hiroaki Yajima

Elisa Misawa

Nobuo Kato

Yasuyoshi Sakai

Kenzo Watanabe

Toshihiro Komeda

Shuji Yanai

Noboru Sakata

Shusaku Hirata

Taizo Goda

Kazunori Nishioka

Mitsunori Iwata

Kosuke Yokozeki

Goro Sakai

Tatsuya Nukushina

Daisuke Fujiwara

Hajime c/o Kirin Beer Kabushiki Kaisha Nozawa

Hideharu Odai

A method for manufacturing a concrete structure comprises an installation step of installing a formworkfor concrete placement, a placement step of performing concrete placement in a state where a curing sheetis attached to an inner surface of the formwork, and a demolding step of removing the formworkafter placement of concrete C. A contact angle of a contact surface of the curing sheetfor use in the placement step with water is 50 degrees or more, wherein the contact surface faces the concrete. Thus, generation of bleeding water is suppressed.

Method for manufacturing concrete structure, concrete curing sheet for curing concrete

Protein synthesis inhibitor resistance genes (typified by a cycloheximide resistance gene) are capable of imparting resistance to a protein synthesis inhibitor (typified by cycloheximide) to animal cells sensitive to the inhibitor. The genes have a sequence mutated by substitution in a gene encoding a ribosome-constituting protein derived from an animal. The genes may be placed in recombinant vectors, including expression vectors containing the gene together with a foreign protein structural gene.

Vectors for animal cells and use thereof

Vectors for animal cells and utilization thereof

The production of carotenoid is accomplished using a DNA molecule that encodes a polypeptide as obtained from or having isopentonyl pyrophosphate (IPP) isomerase activity, or DNA molecule having a nucleotide sequence that hybridizes thereto. In particular, one can introduce such a DNA molecule into a carotenoid-producing microorganism, culture the microorganism thus transformed, and then obtain carotenoids in the culture broth and cells.

Methods of producing carotenoids using DNA molecules encoding isopentenyl pyrophosphate isomerase

An object of the present invention is to provide a vector which can be integrated into a yeast chromosome in a high number of copies. Another object of the present invention is to provide a modified vector which can be integrated into the yeast chromosome in a high number of copies and of which expression units stably maintain on the chromosome. The vector according to the present invention comprises a marker gene for selecting transformants, a shortened promoter sequence which is operably linked to the marker gene and a sequence homologous to the chromosomal DNA of , and optionally a heterologous gene or a gene derived from , wherein the vector is linearized by cleaving within said homologous DNA sequence or at both ends of the homologous DNA sequence with restriction enzymes, and wherein the heterologous gene or the gene derived from can be integrated into the chromosomal DNA of by homologous recombination.

Yeast vector and method of producing proteins using the same

This invention relates to a promoter for dihydroxyacetone synthase gene from ; an expression cassette or vector comprising the promoter and a heterologous gene; a transformant transformed with this vector; and a method for producing an expression product of the heterologous gene which comprises culturing the transformant to express the heterologous gene.

Candida boidini dihydroxyacetone synthase promoter

An object of the present invention is to provide a vector which can be integrated into a yeast chromosome in a high number of copies. Another object of the present invention is to provide a modified vector which can be integrated into the yeast chromosome in a high number of copies and of which expression units stably maintain on the chromosome. The vector according to the present invention comprises a marker gene for selecting transformants, a shortened promoter sequence which is operably linked to the marker gene and a sequence homologous to the chromosomal DNA of,, and optionally a heterologous gene or a gene derived from,, wherein the vector is linearized by cleaving within said homologous DNA sequence or at both ends of the homologous DNA sequence with restriction enzymes, and wherein the heterologous gene or the gene derived from,can be integrated into the chromosomal DNA of,by homologous recombination.

Yeast vector comprising a shortened promoter sequence

Disclosed are the following DNA strands relating to the synthesis of keto group-containing xanthophylls such as astaxanthin and the like, and the techniques relating to the production of xanthophylls by genetic engineering: A DNA strand having a nucleotide sequence which encodes a polypeptide having an enzyme activity for converting a methylene group at the 4-position of a .beta.-ionone ring into a keto group. A DNA strand having a nucleotide sequence which encodes a polypeptide having an enzyme activity for converting a methylene group at the 4-position of a 3-hydroxy-.beta.-ionone ring into a keto group. A DNA strand having a nucleotide sequence which encodes a polypeptide having an enzyme activity for adding a hydroxyl group to the 3-carbon of a 4-keto-.beta.-ionone ring. It is possible to produce a variety of xanthophylls such as canthaxanthin, astaxanthin and the like by introducing the DNA strands into an appropriate microorganism such as Escherichia coli and the like.

DNA strands useful for the synthesis of xanthophylls and the process for

Disclosed are the following DNA strands relating to the synthesis of keto group-containing xanthophylls such as astaxanthin and the like, and the techniques relating to the production of xanthophylls by genetic engineering:

Keto group-introducing enzyme, DNA coding therefor and method for

Transformation systems for the yeast candida utilis and the expression

Growing community of inventors

Kanagawa-ken, Japan

Keiji Kondo