The University of Tokyo

Kawasaki Institute of Industrial Promotion

Transparent Inc.

Accurna, Inc.

Nanocarrier Co., Ltd.

Keiji Itaka

Kazunori Kataoka

Satoshi Uchida

Nobuhiro Nishiyama

Shigeto Fukushima

Naoki Kanayama

Atsushi Harada

Takehiko Ishii

Kanjiro Miyata

Masataka Nakanishi

Yuichi Tei

Takuya Hashimoto

Kensuke Osada

Yuichi Yamasaki

Shourong Wu

Naoto Yoshinaga

Hiroyuki Koyama

Wenjie Wang

Shinsuke Ohba

Ung-il Chung

Woo-Dong Jang

Shunsaku Asano

Hirokuni Uchida

Miyuki Baba

Qixian Chen

Naoki Bamboo-House 203 Kanayama

Atsushi Gransifort-Nakamozu 3-1413 Harada

The present invention provides a functionalized mRNA including mRNA and double-stranded RNA including at least one RNA oligomer hybridized with mRNA. Functionalized mRNA is provided according to this configuration.

MRNA functionalization method

A polyion complex of mRNA and a polycationic polymer, and a composition and a pharmaceutical composition for mRNA delivery are provided. The polyion complex of mRNA and the polycationic polymer can deliver mRNA into cells in the body of a subject almost without inducing inflammation. The polyion complex can then cause mRNA to be uniformly expressed in cells in the body of the subject. The pharmaceutical composition containing the polyion complex is suitable for use in treating a disease whose condition rapidly progresses or an acute disease.

Composition for mRNA delivery

An excellent nucleic acid delivery composition is provided which has reduced cytotoxicity and improved nucleic acid introduction efficiency and gene expression efficiency. The composition comprises: a block copolymer having an uncharged hydrophilic polymer segment and a cationic polymer segment; a cationic polymer; and a nucleic acid, wherein the mol percentage (B/H ratio) of the cationic groups of the block copolymer to the total cationic groups of the block copolymer and the cationic polymer is between 25% and 90%.

Nucleic acid delivery composition and carrier composition, pharmaceutical composition using the same, and method for nucleic acid delivery

The present invention aims to provide a gene delivery system with higher safety and higher sustainability, which is effective for the treatment of ischemic diseases and the like, and the like. The present invention provides a pharmaceutical composition containing, as an active ingredient, a polyanionic substance that suppresses expression of PHD2, and containing a polyion complex of the polyanionic substance and a polycation chargeable polymer.

Polyion complex comprising PHD2 expression inhibiting substance

Facing no ethical obstacle and easily to be isolated, multipotent concretely mesenchymal stem cells (MSCs) are one of the most powerful tools in reconstructive medicine. Here the inventors introduced 3D multicelluar spheroids culture construction based on photolithography and micropatterning techniques to improve multipotent differentiation efficiency of MSCs to adult cells. This invention, the 3D spheroid cultured construction for MSCs, leads to great improve of the differentiation efficiency. This invention relates to a cultured cell construct comprising a support; at least one island on the support; a hydrophilic and cytophobic substance applied on the surface of said support so as to encircle the island; and a spheroid which is derived from MSCs, said spheroid being in contact with the island.

Cultured cell construction containing spheroids of mesenchymal stem cells and utilization thereof

The invention provides block copolymers formed of poly(ethylene glycol) segments and poly(amino acid derivative) segments having side chains of at least one kind of specific amine residue. The invention also provides polyion complexes of such copolymers with polynucleotides and the like. These block copolymers are useful as carriers for in vivo delivery of active substances such as DNA.

Polyethylene glycol/polycation block copolymers

Disclosed is a composition for the delivery of nucleic acid to target cells or tissues, which comprises a polycationically charged polymer as a carrier of nucleic acid. The polycationically charged polymer is a polymer which may comprise a charged polymer segment having a main chain based on poly(amino acid), polysaccharide, polyester, polyether, polyurethane or vinyl polymer and having, as a side chain, a group of formula &#x2014;NH&#x2014;(CH)&#x2014;(NH(CH))&#x2014;NH(wherein a and e independently denote an integer of 1 to 5) which is connected to the main chain either directly or via a linker. The disclosed composition has low toxicity, and has a high efficiency in introducing nucleic acid into cells.

Polycationically charged polymer and the use of the same as a carrier for nucleic acid

Growing community of inventors

Tokyo, Japan

Keiji Itaka