Toyo Boseki Kabushiki Kaisha

Ensuiko Sugar Refining Co., Ltd.

Keiichi Majima

Yoshihisa Kawamura

Koki Fujita

Koji Hara

Sumio Kitahata

Nobuhiro Kuwahara

Yuzo Hayashi

Shigeki Asano

Shin-ichi Teshima

A reagent for amylase isozyme activity assay, which contains 2-chloro-4-nitrophenyl 4-O-&bgr;-D-galactopyranosylmaltoside, an antibody inhibiting S-type amylase activity or P-type amylase activity, and an amylase activator, and methods for assaying amylase isozyme activity using the regent is provided. According to the present invention, amylase isozyme activity can be accurately and easily assayed using a low concentration activator and an antibody having S-type amylase or P-type amylase activity inhibitory action without using an adjuvant enzyme.

Reagent for assay of amylase isozyme activity

A reagent for determining a-amylase activity, comprising a maltooligosaccharide derivative of the following formula ##STR1## (wherein either one of R.sub.1 and R.sub.2 is .beta.-galactopyranosil and the other is hydrogen, R.sub.3 is a group bonded to the reducing terminal glucose via a bond cleavable by .alpha.-amylase, which becomes a measurable substance upon cleavage of the bond, and n is an integer of 0-2), which does not comprise adjuvant enzymes; and a method for determining .alpha.-amylase activity which comprises use of the reagent. The reagent of the present invention does not require use of any adjuvant enzyme and is stable since the substrate is not exposed to the decomposition by an adjuvant enzyme. The substrate used in the present invention has high affinity for .alpha.-amylase. Thus, the reagent and the determination method of the present invention make it possible to determine .alpha.-amylase activity with high sensitivity.

Reagent for Determining .alpha.-amylase activity and method for

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Tsuruga, Japan

Keiichi Majima