Novozymes A/s

Novo Zymes North America, Inc

Keiichi Ayabe

Guillermo Coward-Kelly

Sara Maria Landvik

Shiro Fukuyama

Marc Dominique Morant

Noriko Tsutsumi

Esben Peter Friis

Tomoko Matsui

Aki Tomiki

Li Wang

Zheng Liu

Junxin Duan

Jens Erik Nielsen

Randall Scott Deinhammer

Roland Alexander Pache

Yuma Kurakata

Allan Svendsen

Jesper Vind

Frank Winther Rasmussen

Peter Kamp Hansen

Mary Ann Stringer

Hanne Vang Hendriksen

Joyce Craig

Steen Krogsgaard

Michael Skjøt

Mikako Sasa

Thomas Agsersten Poulsen

Tomoko Namoto

Sara Landvik

Zhengfang Kang

Takashi Nakanishi

Guillermo Coward Kelly

Anne Mette Bhatia Frederiksen

Siik Kishishita

Ming Li

Seiichiro Kishishita

The present invention relates to protease variants, having improved properties compared to the parent protease, in particular variants of a serine protease belonging to family 53 derived from a strain of. The variants according to the invention have in particular increased thermo-stability, e.g., increased residual activity after 30 min at a temperature in the range from 55 to 60° C. and/or increased thermal denaturation temperature, compared to the parentprotease. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Serine protease variants and polynucleotides encoding same

The present invention relates to a method of making glucose syrup from liquefied starch comprising, (a) contacting the liquefied starch with a glucoamylase, a pullulanase, and optionally an alpha-amylase wherein the ratio of pullulanase dose expressed as NPUN/gDS, to alpha-amylase dose expressed as FAU(A)/gDS is at least 60, particularly at least 75, particularly at least 100, more particularly at least 150, more particularly at least 200, more particularly at least 250, more particularly at least 300, more particularly at least 400, more particularly at least 500, more particularly at least 600, more particularly at least 800 or if no alpha-amylase is present the pullulanse is present in a dose of at least 0.5, particularly at least 0.75, particularly at least 1.0, particularly at least 1.5 NPUN/gDS, and (b) saccharifying the liquefied starch.

Compositions for producing glucose syrups

The present invention relates to protease variants, having improved properties compared to the parent protease, in particular variants of a serine protease belonging to family 53 derived from a strain of. The variants according to the invention have in particular increased thermo-stability, e.g., increased residual activity after 30 min at a temperature in the range from 55 to 60&#xb0; C. and/or increased thermal denaturation temperature, compared to the parentprotease. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

The present invention relates to a hybrid polypeptide having alpha-amylase activity, selected from a first polypeptide sequence comprising a catalytic core, and a second polypeptide sequence comprising a carbohydrate binding module (CBM), wherein (a) the catalytic core is selected from a polypeptide having at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to amino acids 20 to 494 of SEQ ID NO: 1 or amino acids 20 to 496 of SEQ ID NO: 1; and (b) the CBM is selected from a polypeptide having at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 4, SEQ ID NO: 5, or SEQ ID NO: 6. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and catalytic domains.

Polypeptides having alpha-amylase activity and polynucleotides encoding same

The present invention relates to glucoamylase variants having an increase in raw starch activity compared to the guy-coamylase disclosed as SEQ ID NO: 2, comprising one or more modifications in the catalytic domain and/or one or more modifications in the starch binding domain selected from: a) at least one, preferably at least two, preferably at least three, preferably at least four of: V18M, T43K, N112L, T116R, A117Q, G120S, A271F, Y295W, Q318Y; and/or b) Introducing at least three, preferably at least four substitutions selected from the group: S458C, S458SCGG, S458SGGC, A493V, A518K, E520Q, N527M, S540K, R, S(G)546P, T(V)549W, N503R, N539R+I541Y+T543V+A545S+S546GCGV+G547S+S548T+T549A. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Glucoamylase variants and polynucleotides encoding same

The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Polypeptides having glucoamylase activity and polynucleotides encoding same

The present invention relates to method for production of brewer's wort comprising adding to a mash, a particular glucoamylase. The glucoamylase is obtained from. Further, the invention relates to use of a particular glucoamylase for production of brewer's wort. Furthermore, the invention relates to use of a combination of a glucoamylase and a pullulanase for production of brewer's wort.

Method for production of brewers wort

The present invention relates to glucoamylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

The present disclosure relates to isolated polypeptides having alpha-amylase activity, polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing the polypeptides, and method of using polypeptides, including in ethanol production processes.

Alpha-amylases and polynucleotides encoding same

The present invention relates to asparaginase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. The invention further relates to a process of producing a fermentation product, comprising: liquefying a starch-containing material to dextrins with an alpha-amylase in the presence of an asparaginase of the invention; saccharifying the dextrins to a sugar with a glucoamylase; and fermenting the sugar using a fermenting organism.

Thermostable asparaginase variants and polynucleotides encoding same

The present invention relates to variants of a parent glucoamylase. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the glucoamylase variants.

Glucoamylase variants and polynucleotides encoding same and uses thereof

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Chiba, Japan

Keiichi Ayabe