Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo

Kei Suzuki

Toshio Miyake

Masaru Yoneyama

Yukio Suzuki

Hiromi Hijiya

A novel glycoside, 4.sup.G -alpha-D-glucopyranosyl rutin, is formed by a saccharide-transferring enzyme and glucoamylase in a solution which contains rutin together with glucoamylase. The 4.sup.G -alpha-D-glucopyranosyl rutin formed in such a solution is purified with a synthetic macroreticular resin, and crystallization in an organic solvent yields a complex crystal with the organic solvent. 4.sup.G -Alpha-D-glucopyranosyl rutin exhibits the same molecular absorption coefficient as intact rutin, and is readily water-soluble, substantially tasteless and odorless, and readily hydrolyzable in vivo to exhibit physiological activities inherent to rutin. These render 4.sup.G -alpha-D-glucopyranosyl rutin very useful as a highly-safe, natural yellow coloring agent, antioxidant, stabilizer, quality-improving agent, preventive, remedy, uv-absorbent and deterioration-preventing agent in foods, beverages, tobaccos, cigarets, feeds, pet foods, pharmaceuticals, cosmetics and plastics.

4.sup.G -alpha-D-glucopyranosyl rutin, and its preparation and uses

Alpha-glycosyl rutin is formed at a high concentration by allowing a saccharide-transferring enzyme to act on a high-rutin content liquid in suspension or solution to effect saccharide-transfer reaction. The resultant alpha-glycosyl rutin is easily recovered from the reaction mixture by allowing it to contact with a synthetic macroreticular resin. Alpha-glycosyl rutin is superior in water-solubility, resistance to light and stability to intact rutin, as well as having the physiological activities as intact rutin has. Thus, alpha-glycosyl rutin is favorably usable as a yellow coloring agent, antioxidant, stabilizer, fading-preventing agent, quality-improving agent, preventive, remedy, uv-absorbent and deterioration-preventing agent in foods, beverages, tobaccos, cigarettes, feeds, pet foods, pharmaceuticals for susceptive diseases, cosmetics including skin-refining agent and skin-whitening agent, and plastics, in addition to the use in vitamin P-enriching agents.

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Okayama, Japan

Kei Suzuki