Takara Shuzo Co., Inc.

Takara Bio Inc.

Kei Fujinaga

Fumihiro Higashino

Koichi Yoshida

Ikunoshin Kato

Kiyozo Asada

Mitsuhiro Ueno

Takashi Ueno

Hideto Chono

Kimikazu Hashino

Mutsumi Sano

Mio Morishita

Hirofumi Yoshioka

Keiji Tanaka

Tsuyoshi Miyamura

Hajime Matsumura

Tomoko Iwasaki

Haruko Konishi

Clarification of the relationship between the functions of E1AF gene and the invasion and metastasis of cancers, and application thereof to cancer control, more specifically the control of cancer cell invasion by controlling the expression of E1AF gene and the products of expression, and the detection and diagnosis of cancers by detecting the products of expression of E1aF gene.

Cancer control

A method of transferring a foreign gene into cells, characterized by involving: the step of transferring into the cells with the use of an adenovirus vector, a first nucleic acid, which has a sequence provided with adeno-associated virus-origin ITRs in both sides of the target foreign gene to be transferred, and a second nucleic acid, which has an adeno-associated virus-origin rep gene and a promoter for expressing this gene and carries a stuffer sequence inserted thereinto sandwiched in two recombinase recognition sequences and located between the rep gene and the promoter; and the step of expressing the Rep protein under the action of recombinase in the cells obtained in the above step to thereby integrate the target foreign gene into the chromosomal DNA.

Gene transfer method

Improved methods for transferring a gene into target cells by using a retrovirus, wherein the gene transfer efficiency is improved and the target cells are efficiently transformed by binding the retrovirus to a functional substance which is immobilized on as carrier and having an activity of binding to retroviruses followed by washing; using an antibody capable of specifically recognizing cells, laminin or mannose-rich type sugar chain as a substance having an activity of binding to the target cells; pre-treating the target cells so as to inactivate transferrin receptor, or introducing a new functional group into the functional substance.

Gene transfer methods

Growing community of inventors

Sapporo, Japan

Kei Fujinaga