Kyoto University

Massachusetts Institute of Technology

Ips Academia Japan, Inc.

Kei Endo

Darrell J Irvine

Ron Weiss

Yoshinori Yoshida

Hirohide Saito

Kenji Miki

Tan Inoue

Seiya Takahashi

Tasuku Kitada

Liliana Wroblewska

Maria Hottelet Foley

Katie Bodner

Callum Parr

Velia Siciliano

Tyler Wagner

Jacob Becraft

Shota Katayama

Engineered synthetic RNA-based genetic circuits are provided that are regulated exclusively at the post-transcriptional level.

RNA-based logic circuits with RNA binding proteins, aptamers and small molecules

A method for extracting differentiated cells from a cell population comprising undifferentiated cells after induction of the differentiation of pluripotent stem cells. A method for extracting differentiated cells from a cell population, comprising the following steps: (1) a step of introducing, into a cell population, mRNA comprising a marker gene operably linked to the target sequence of miRNA specifically expressed in pluripotent stem cells; and (2) a step of extracting cells in which the marker gene has been translated.

Method for extracting differentiated cells

A method for distinguishing living cells in a living state with high accuracy. A method for distinguishing a desired cell type from a cell group comprising two or more types of cells, using the expression of miRNA as an indicator, wherein the method comprises the following steps:

Method for determining desired cell type using expression of miRNA as indicator

An object of the present invention is to provide a novel method for sorting cardiomyocytes. Another object of the present invention is to provide a method for producing high-purity cardiomyocytes and a kit used therefor. The present invention provides a method for sorting cardiomyocytes, comprising a step of introducing miRNA-responsive mRNA into a cell group, wherein the miRNA-responsive mRNA consists of a sequence comprising the following (i) and (ii):

Method for sorting cardiomyocytes

An object of the present invention is to provide a method for increasing the purity of a type of tissue cell such as an endothelial cell, a hepatocyte, or an insulin-producing cell. The present invention solves the problem by providing a method comprising a step of introducing, into a cell population, an mRNA comprising a nucleic acid sequence recognized by an miRNA specifically expressed in endothelial cells, hepatocytes, or insulin-producing cells.

Method for sorting tissue cells

A translational control method using an RNA-protein interaction motif is provided. The method comprises a step of introducing an mRNA having: a 5'UTR regulation structure comprising: (1) a cap structure at the 5&#x2032; terminus, (2) a spacer positioned on the 3&#x2032; side of the cap structure, and (3) one or more RNA motifs positioned on the 3&#x2032; side of the spacer, which comprises an RNA-protein interaction motif-derived nucleotide sequence or a variant thereof; and a nucleotide sequence encoding a target protein gene on the 3&#x2032; side of the 5&#x2032;UTR regulation structure, into a cell in the presence of a protein specifically binding to the RNA motifs, wherein a translational level is decreased as the number of bases of the spacer decreases, and the translational level is decreased as the number of the RNA motifs increases.

Translational control system using RNA-protein interaction motif

Growing community of inventors

Kyoto, Japan

Kei Endo