Shandong Lonct Enzymes Co. Ltd

Tianjin University of Science and Technology

Kefen Wang

Xingji Wang

Wenlong Liu

Fuping Lu

Yu Li

Fufeng Liu

Yihan Liu

Huitu Zhang

Jie Zhang

Xinwei Tong

An alkaline protease mutant, and a gene, engineered strain, a preparation method and application thereof are provided. The method comprises the following steps of extracting genome DNA of, performing PCR amplification to obtain a wild-type alkaline protease gene sequence, mutating the wild-type alkaline protease gene obtained by the amplification through an error-prone PCR, performing high-throughput screening to obtain a plurality of highly active alkaline protease genes, performing DNA shuffling on the highly active alkaline protease genes, and performing screening to obtain eight alkaline protease mutant genes with higher activity.

Alkaline protease mutant, and gene, engineered strain, preparation method and application thereof

Glucoamylase mutant GA3 with improved specific activity and thermal stability, and its gene and application are provided, belonging to the field of gene engineering. The glucoamylase mutant GA3 of the present invention obtained by sequentially mutation at fixed points starting from wild glucoamylase processes both improved thermal stability and catalytic efficiency, and can be applied to the industries of feed, food, medicine and the like.

Glucoamylase mutant GA3 with improved specific activity and thermal stability, and gene and application thereof

Growing community of inventors

Linyi, China

Kefen Wang