Sekisui Chemical Co., Ltd.

Kazuyuki Oishi

Toshiki Kawabe

Izumi Omoto

Kazuhiko Shimada

Eriko Kusaka

Yuji Setoguchi

Takayuki Oka

An object of the present invention is to provide a method for measuring hemoglobin that enables short-time, high-accuracy measurement of hemoglobin, in particular stable hemoglobin A1c, which is used as a diagnostic indicator of diabetes mellitus, and an electrophoresis apparatus that is suitably used in this measurement method. The present invention provides a method for measuring hemoglobins using electrophoresis, which includes: using a migration path having an inner surface coated with a cationic substance to be immobilized on the inner surface or a migration path having an inner surface made of a cationic material; and using a buffer solution containing a water-soluble polymer having an anionic group as an electrophoresis buffer solution.

Hemoglobin measurement method and electrophoresis apparatus

An object of the present invention is to provide a method for measuring hemoglobin using electrophoresis, in particular a method for measuring hemoglobin that enables high accuracy measurement of stable hemoglobin A1c and a method for simultaneously measuring stable hemoglobin A1c and abnormal hemoglobins. The present invention provides a method for measuring hemoglobin using electrophoresis, which comprises: immobilizing an ionic polymer on an inner surface of a migration path; and using a buffer solution containing a sulfated polysaccharide.

Hemoglobin determination method

A method for separating hemoglobin A from a hemoglobin mixture according to a cation exchange liquid chromatography characterized that at least two types of eluents are used including an eluent (hereinafter referred to as &ldquo;eluent A&rdquo;) for elution of faster hemoglobins than hemoglobin A and an eluent (hereinafter referred to as &ldquo;eluent B&rdquo;) for elution of hemoglobin A and slower hemoglobins than hemoglobin A , and that the eluent A has a pH of 4.0-6.0 and the eluent B is maintained at a pH of at least 0.5 higher than that of the eluent A.

Method of separating hemoglobin A2

A method for determining hemoglobins is provided which is suitable for determination of stable hemoglobin A . A method for determining hemoglobins by cation exchange liquid chromatography wherein an eluent is used containing a chaotropic ion and further an inorganic acid, an organic acid and/or any salt thereof having a buffer capacity in the 4.0-6.8 pH range.

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Osaka, Japan

Kazuyuki Oishi