Kaneka Corporation

Kanegafuchi Kagaku Kogyo Kabushiki Kaisha

Kanegafuchi Chemical Industry Co., Ltd.

Kazuyoshi Yajima

Yasuhiro Ikenaka

Satomi Takahashi

Masayuki Takano

Yukio Yamada

Hirokazu Nanba

Hideyuki Matsuda

Makoto Kawamukai

Yasuyoshi Ueda

Shiro Kitamura

Takahisa Kato

Akihisa Kanda

Sakayu Shimizu

Hideaki Yamada

Junzo Hasegawa

Akihiko Kosugi

Takehisa Ohashi

Shinichi Yokota

Tetsu Kakutani

Akiko Nishi

Kazuhiko Yamada

Yoshiro Hiraishi

Keiji Matsumoto

Kenichi Nishi

Takumi Mano

Tetsuya Nagaoka

Hitoshi Yahara

Yasuhiro Inenaka

Kazuma Okubo

A process for producing on an industrial scale the oxidized coenzyme Q, includes culturing a reduced coenzyme Q-producing microorganism selected from the group consisting of the genus, the genus, the genusand the genus, to obtain microbial cells containing reduced coenzyme Qat a ratio of not less than 70 mole % among the entire coenzymes Q; and one of: (a) oxidizing thus-obtained reduced coenzyme Qto oxidized coenzyme Qand then extracting the oxidized coenzyme Qby an organic solvent; or (b) extracting reduced coenzyme Qby an organic solvent and oxidizing the extracted reduced coenzyme Qto oxidized coenzyme Q.

Process for producing coenzyme Q10

The present invention relates to a process for producing reduced coenzyme Qwhich comprises obtaining microbial cells containing reduced coenzyme Qat a ratio of not less than 70 mole % among the entire coenzymes Q, optionally disrupting the cells and recovering thus produced reduced coenzyme Q. The present invention also relates to a process for producing oxidized coenzyme Qwhich comprises either recovering oxidized coenzyme Qafter oxidizing the above-mentioned microbial cells or disrupted product thereof, or recovering reduced coenzyme Qfrom the above-mentioned microbial cells or disrupted product thereof to oxidize thus-obtained reduced coenzyme Qthereafter. According to the processes of the present invention, reduced coenzyme Qand oxidized coenzyme Qcan be produced simply on the industrial scale.

[object Object]

The present invention relates to an efficient and economical process for producing a protein A-like protein. Hosts such asandhave been used in the production of a protein A-like protein using a genetic recombination technique and however, their low productivity has been a big cause of high cost. Thus, it has been desired strongly to immediately establish a technique enabling the inexpensive, large-scale production of a protein A-like protein using recombinant DNA techniques other thanand. The present invention provides a process for producing a protein A-like protein in large amounts, for example, a process comprising allowing a recombinantgenus bacterium to express and secrete the protein in large amounts into a culture solution and separating and collecting the accumulated protein A-like protein from the culture solution.

The present invention relates to an efficient and economical process for producing a protein A-like protein. Hosts such asandhave been used in the production of a protein A-like protein using a genetic recombination technique and however, their low productivity has been a big cause of high cost. Thus, it has been desired strongly to immediately establish a technique enabling the inexpensive, large-scale production of a protein A-like protein using recombinant DNA techniques other thanandThe present invention provides a process for producing a protein A-like protein in large amounts, for example, a process comprising allowing a recombinantgenus bacterium to express and secrete the protein in large amounts into a culture solution and separating and collecting the accumulated protein A-like protein from the culture solution.

The present disclosure describes a method of producing a long-chain prenyl diphosphate synthase (in particular decaprenyl diphosphate synthase and solanesyl diphosphate synthase) using a gene and a protein which are required for enabling or enhancing the activity expression of a eukaryote-derived long-chain prenyl diphosphate synthase as well as a method of efficiently producing a coenzyme Q having a long-chain isoprenoid in its side chain (in particular coenzyme Qor coenzyme Q) using a microorganism. The present disclosure also describes a DNA having a base sequence shown under SEQ ID NO:1, 3 or 5 and a DNA sequence derived from the above base sequence by deletion, addition, insertion and/or substitution of one to several bases thereof, and coding for a protein enabling (functioning) or enhancing the activity expression of a eukaryote-derived long-chain prenyl diphosphate synthase in a cell of a host organism.

Method of expressing long-chain prenyl diphosphate synthase

The present invention relates to a process for producing reduced coenzyme Qwhich comprises obtaining microbial cells containing reduced coenzyme Qat a ratio of not less than 70 mole % among the entire coenzymes Q, optionally disrupting the cells and recovering thus-produced reduced coenzyme Q. The present invention also relates to a process for producing oxidized coenzyme Qwhich comprises either recovering oxidized coenzyme Qafter oxidizing the above-mentioned microbial cells or disrupted product thereof, or recovering reduced coenzyme Qfrom the above-mentioned microbial cells or disrupted product thereof to oxidize thus-obtained reduced coenzyme Qthereafter. According to the processes of the present invention, reduced coenzyme Qand oxidized coenzyme Qcan be produced simply on the industrial scale.

Processes for producing coenzyme Q10

The present invention provides a method of producing a long-chain prenyl diphosphate synthase (in particular decaprenyl diphosphate synthase and solanesyl diphosphate synthase) using a gene and a protein which are required for enabling or enhancing the activity expression of a eukaryote-derived long-chain prenyl diphosphate synthase as well as a method of efficiently producing a coenzyme Q having a long-chain isoprenoid in its side chain (in particular coenzyme Qor coenzyme Q) using a microorganism. The present invention relates to a DNA having a base sequence shown under SEQ ID NO:1, 3 or 5 and a DNA sequence derived from the above base sequence by deletion, addition, insertion and/or substitution of one to several bases thereof, and coding for a protein enabling (functioning) or enhancing the activity expression of a eukaryote-derived long-chain prenyl diphosphate synthase in a host microorganism.

The invention aims at providing a process for producing coenzyme Qefficiently in microorganisms by utilizing a coenzyme Qside chain synthesis gene derived from a fungal species belonging to the genusand genus The present invention relates to a DNA having a DNA sequence described under SEQ ID NO:1 and 2 or derived from the above sequence by deletion, addition, insertion and/or substitution of one or several bases and encoding a protein having decaprenyl diphosphate synthase activity.

The subject of the present invention is to provide a &#x3b2;-lactam acylase protein having high activity, a gene coding for said &#x3b2;-lactam acylase protein, a recombinant vector having said gene, a transformant containing said recombinant vector, and a method of producing a &#x3b2;-lactam antibiotic such as amoxycillin using said &#x3b2;-lactam acylase. A &#x3b2;-lactam acylase gene ofwas cloned, the DNA base sequence and the amino acid sequence expected therefrom was determined, and a&#x3b2;-lactam acylase gene was obtained. This gene was found to code for a protein with a molecular weight of about 70 kDa and having &#x3b2;-lactam acylase activity, and could efficiently produce amoxycillin without being inhibited by phenylacetic acid, etc. Furthermore, by modification of the amino acid sequence, a protein which can more efficiently produce amoxycillin could be obtained.

Acylase gene

A gene of decaprenyl diphosphate synthase, which is the key gene participating in the biosynthesis of coenzyme Q was isolated from a bacterium belonging to the family Rhizobiaceae. By transferring this gene into a microorganism such as and expressing therein, coenzyme Q can be effectively produced.

A DNA fragment coding for a decarbamylase protein improved in thermostability as the result of replacement of at least one base of a DNA fragment coding for a decarbamylase protein derived from a microorganism with another base and the resultant replacement of at least one of the corresponding amino acids, and its production process; a vector containing the DNA fragment; a transformant obtained by transformation with the vector; as well as a decarbamylase improved in thermostability and its production process. Also disclosed is a process for producing a D-.alpha.-amino acid, which comprises converting an N-carbamoyl-D-.alpha.-amino acid into the corresponding D-.alpha.-amino acid in an aqueous medium by the action of a decarbamylase having a thermostable temperature of 65.degree. C. or higher; and collecting the D-.alpha.-amino acid produced.

DNA coding for decarbamylase improved in thermostability and use thereof

Process for producing D-amino acids with composite immobilized enzyme

Process for the production of D-.alpha.-amino acids by hydrolysis of the

In a process for the production of a D-.alpha.-amino acid, in which an N-carbamyl-D-.alpha.-amino acid corresponding to the general formula: ##STR1## wherein R represents phenyl, hydroxy-substituted phenyl, substituted or unsubstituted alkyl, or thienyl, is converted by a microbial enzyme in an aqueous medium to a D-.alpha.-amino acid corresponding to the general formula: ##STR2## wherein R is the same as defined above, decarbamylase produced by a microorganism of the genus Comamonas, Blastobacter, Alcaligenes, Sporosarcina, Rhizobium, Bradyrhizobium or Arthrobacter is used as the enzyme converting the N-carbamyl-D-.alpha.-amino acid to the D-.alpha.-amino acid. The conversion of the N-carbamyl-D-.alpha.-amino acids to the D-.alpha.-amino acids is carried out in a neutral to alkaline pH range.

Growing community of inventors

Hyogo, Japan

Kazuyoshi Yajima