Brookhaven Science Associates, Lcc

Kazuyasu Sakaguchi

Carl W Anderson

Ettore Appella

The present invention provides methods for generating phosphorylation site-specific immunological reagents. More specifically, a phosphopeptide mimetic is incorporated into a polypeptide in place of a phosphorylated amino acid. The polypeptide is used as antigen by standard methods to generate either monoclonal or polyclonal antibodies which cross-react with the naturally phosphorylated polypeptide. The phosphopeptide mimetic preferably contains a non-hydrolyzable linkage from the appropriate carbon atom of the amino acid residue to a phosphate group. A preferred linkage is a CF,group. Such a linkage is used to generate the phosphoserine mimetic F,Pab, which is incorporated into a polypeptide sequence derived from p53 to produce antibodies which recognize a specific phosphorylation state of p53. A CF,group linkage is also used to produce the phosphothreonine mimetic F,Pmb, and to produce the phosphotyrosine mimetic, F,Pmp.

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Montgomery, MD, United States of America

Kazuyasu Sakaguchi