Nara Institute of Science and Technology

Idemitsu Kosan Company, Limited

Obihiro University of Agriculture and Veterinary Medicine

National University Corporation Nara Institute of Science and Technology

National University Corporation Obihiro University of Agriculture and Veterinary Medicine

Kazuya Yoshida

Ko Kato

Takeshi Matsui

Kazutoshi Sawada

Atsuhiko Shinmyo

Yoshikazu Tanaka

Takaaki Kusumi

Sou-ichi Makino

Michio Shibata

Shingo Nagaya

Keiko Kawamoto

Ryutaro Aida

Koji Akasaka

Sou-Ichi Makino

A technology for producing a pig edema disease vaccine at low cost and at high efficiency is developed. Specifically, a gene of a pig edema disease toxin protein (Stx2e protein) is efficiently expressed in plant cells to produce a plant vaccine for pig edema disease at low cost. An Stx2e protein including a secretory signal peptide derived from a plant added at an amino terminus is expressed in cells of a plant such asusing the 5'-untranslated region of an alcohol dehydrogenase gene (ADH5&#x2032;UTR) derived from a plant.

Transgenic plants expressing STX2EB protein for use as a pig edema disease vaccine

A bacterial toxin protein such as a Shiga toxin protein is efficiently produced using plant cells. The plant cells are transformed using a DNA construct containing DNA encoding a hybrid protein in which the bacterial toxin proteins such as the Shiga toxin proteins are tandemly linked through a peptide having the following characteristics (A) and (B) to produce the bacterial toxin protein in the plant cells: (A) a number of amino acids is 12 to 30; and (B) a content of proline is 20 to 35%.

Bacterial toxin vaccine

A method useful for stable expression of an exogenous gene, introduced into a plant, is provided. By introducing an exogenous gene and 5&prime; upstream sequence of tobacco dehydogenase concurrently, stable expression of the exogenous gene introduced into a plant can be achieved.

DNA fragment for stable expression of an exogenous gene in a plant

A method for the stable expression of an introduced exogenous gene in a plant or plant cell is provided. Stable expression of an exogenous gene that was introduced was achieved by operably linking an upstream sequence of sea urchin arylsulfatase gene as an insulator.

Method of stable gene expression in a transgenic plant utilizing an insulator nucleotide sequence from the sea urchin arylsulfatase gene

Growing community of inventors

Nara, Japan

Kazuya Yoshida