Takara Bio Inc.

Kazuya Matsumoto

Ikunoshin Kato

Junichi Mineno

Hideto Chono

Kazutoh Takesako

Hirofumi Yoshioka

Masao Funakoshi

Yasushi Katayama

Hajime Matsumura

Daisuke Tomura

A nucleic acid comprising a transcription regulation sequence whose transcription is induced by a trans-acting factor of a human immunodeficiency virus and a gene encoding a polypeptide having an endoribonuclease activity specific to single-stranded RNA, wherein the gene is located in such a position that the expression of the gene can be regulated by the transcription regulation sequence; a method for production of a cell showing an inhibited replication of a human immunodeficiency virus therein, the method comprising the step of introducing the nucleic acid into a cell; and a method for treatment or prevention of a human immunodeficiency virus infection.

Nucleic acid for treatment or prevention of immunodeficiency virus infection

A method for obtaining a virus vector free of a serum, and a serum-free medium for cultivation of a virus producer cell which can be used for the method are provided. By cultivating a virus producer cell using a serum-free medium containing serum albumin, it is possible to cultivate the cell in a state equivalent to that in a serum-containing medium to produce a virus vector at a sufficient titer. The virus vector prepared from the virus producer cell cultivated in the medium exhibits gene transfer efficiency comparable to that of a conventional vector. The medium is useful for gene therapy and studies thereof.

Serum-free medium for producing retroviruses

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Otsu, Japan

Kazuya Matsumoto