Fuji Yakuhin Kogyo Kabushiki Kaisha

Kazushi Iwata

Akira Oshima

Taro Hayakawa

Yasuo Bai

Shinichi Yoshida

Yasuteru Muragaki

Shuji Kodama

Kyoko Yamashita

Hisashi Iwata

Eiji Ishikawa

Miki Miyagiwa

Eisaku Matsumoto

Kyoichi Inoue

Satoshi Miyamoto

Junichi Kishi

Yasuo Nagai

Kenichi Obata

Takafumi Ichida

Kazuyoshi Inada

Provided are serum-free media for tissue culture containing tissue inhibitor of metalloproteinases and methods for culturing animal cells using these media.

Serum-free medium for tissue culture containing tissue inhibitor of

A method for determining human type III, IV and VI collagen peptides by way of enzyme immunoassay according to the sandwich technique using a monoclonal antibody and a polyclonal antibody each to the human type III, IV or VI collagen peptide, characterized in that the monoclonal antibody to the collagen peptide is used as an antibody to be coated onto a solid phase and/or an antibody to be labeled with an enzyme. Also disclosed is a sandwich technique using two different monoclonal antibodies. This method can be carried out with a smaller amount of samples in a simple manner to obtain a precise result and is thus useful for diagnosis of hepatic diseases.

Method for determining human collagen peptides by way of enzyme

Method for the diagnosis of rheumatoid arthritis

Method for the diagnosis of hepatic carcinoma

The present invention is directed to a method for detecting hepatic diseases which are associated with fibrosis by determining the level of human prolyl hydroxylase in a serum sample which comprises:

Method for determining beta-subunit of human prolyl 4-hydroxylase by

A method for determining human prolyl hydroxylase by radioimmunoassay according to the sandwich method wherein a monoclonal antibody to human prolyl hydroxylase and polyclonal antibody to human prolyl hydroxylase are used, characterized in that a monoclonal antibody to human prolyl hydroxylase is used as at least one of the antibodies which are to be coated on a solid support and to be labeled with a radioactive element. This method is simple and operable with small amounts of samples and gives exact results. Thus, this method is useful for the diagnosis of hepatic diseases. A monoclonal antibody specific to the .beta.-subunit of human prolyl hydroxylase is used to test for the human prolyl hydroxylase.

Method for determining the .beta.-subunit of human prolyl hydroxylase by

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Takaoka, Japan

Kazushi Iwata