Hitachi, Ltd.

On-chip Biotechnologies Co., Ltd.

Hitachi Displays, Ltd.

Other

Hiatchi, Ltd.

Shizuoka Prefecture

Hitachi Industries Co., Ltd.

Fujikasui Engineering Co., Ltd.

Kazuo Takeda

Masakazu Saito

Shigeru Matsui

Makoto Ohkura

Muneo Maeshima

Yuu Fujimura

Takeo Shiba

Haruo Miura

Yoshitaka Kodama

Hitoshi Komuro

Koji Tomita

Masayuki Ishige

Takahide Ino

Takeshi Sato

Yoshitoshi Ito

Jun Gotoh

Kosuke Osawa

Yoshinobu Kimura

Hiroshi Fukuda

Kenji Yasuda

Shin-ichiro Umemura

Yukio Takasaki

Koji Kotani

Hironobu Abe

Chusuke Munakata

Takeshi Satou

Yoshinori Harada

Tadashi Kaneki

Yuji Morishita

Soichiro Tsuda

Fumie Jimma

Masashi Takao

Jun Goto

Jun Gotou

Jin Akagi

Kenichi Kawabata

Takanori Ninomiya

Kiyoshi Ogata

Aritoshi Sugimoto

Masao Kamahori

Yasuhiro Mitsui

Hiromi Kobayashi

Atsushi Hiraiwa

Hiroshi Takeuchi

Keiichi Nagai

Kenko Uchida

Fumio Kawaguchi

Hideo Nishida

Yuichi Yamashita

Naohiko Takahashi

Norio Shimizu

Takuo Tamura

Masahiro Yoshioka

Fumiaki Koizumi

Masaru Watanabe

Hirofumi Shimizu

Hironaru Yamaguchi

Kazuaki Sasaki

Yasushi Matsuda

Seiichi Isomae

Daisuke Mutou

Isao Nemoto

Noriaki Honma

Hiromichi Shimizu

Toshio Itou

Hidetsugu Ishida

Mitsuru Tamura

Namiko Yamashita

Yuri Uehara

Tadayoshi Funakoshi

Yohsuke Bansho

Naoki Fujihata

Katsuo Oikawa

Takayoshi Harimoto

Kaori Nishio

Toshiaki Matsuoka

Akihiko Hongyou

Kouji Shiraishi

Tomoyuki Ishikawa

Yasuhiro Koh

Kyoko Minowa

One object of the present invention is to provide a method or apparatus for purifying target particles from high-concentration particles in a short time. The above problem can be solved by a method for purifying target particles, characterized in that the method comprises a step of sorting the target particles from a high concentration of non-target particles, wherein the sorting step is repeated for three times or more.

Method for purifying particles, method for dispensing a single particle, method for analyzing cell cluster, and apparatus using the same

The object of the present invention is to provide a technique for dispensing cells as single cells. The above problem can be solved by an apparatus for dispensing particles comprising a transparent hollow pipette for dispensing sample liquid containing particles, and an image capturing means, wherein the devise has means for capturing two or more images of the dispensing liquid in the hollow pipette; means for comparing two or more shot images, distinguishing a moved particle-like substance from an unmoved particle-like substance in the image-captured particle-like substances, and identifying the moved particle-like substance as a suspended particle; and means for dispensing a sample liquid containing a target number of suspended particles.

Fine particle dispensing device, fine particle analysis device, reaction detection device, and method using said devices

An object of the present invention is to purify and concentrate differentiating cells derived from ES cells, iPS cells, or the like without damaging them. The above problem can be solved by an apparatus for analyzing and separating particles comprising: a flow path cartridge, an illumination unit, a detection unit for detecting particles of interest, a force generating unit, wherein a sample liquid reservoir (sample reservoir) connected to a first flow path; a fourth branched flow path and a fifth branched flow path which are connected to the first flow path; a third-A reservoir connected to the fourth branched flow path; a third-B reservoir connected to the fifth branched flow path; and a fourth reservoir for reserving particles which are not sorting; are formed on the cartridge, and each reservoir comprise a means which equalizes an air pressure in the each reservoir with an air pressure of an in-device air pressure control system, and a stream of the flow path in the cartridge is controlled by controlling the air pressure in the each reservoir through the each in-device air pressure control system.

Single-particle analysis method, and system for performing said analysis

An apparatus for analyzing particles in a solution includes a unit configured to place a flow cell having a flow path for flowing a sample solution containing the particles; a unit configured to illuminate the sample solution flowing through the flow path of the flow cell; a photodetector that detects a scattered light and/or fluorescence generated from the particles in the sample solution; and a unit configured to analyze the particles based on their signal intensities detected by the photodetector, wherein the flow cell has the flow path formed in a substrate, a reflection plane is formed on the side surface of the flow path, the reflection plane leads the lights generated in the flow path of the flow cell and advancing in the substrate in-plane direction to a specified region of the surface of the flow cell, and the photodetector detects the light exiting from the specified region to the outside.

Apparatus and method for analyzing and sorting cell particles in solution

The object of the present invention is to provide an evaluation method capable of accurately determining a metastasis of cancer, the stage of cancer progression, or the malignancy of cancer. The object can be solved by a method for detecting the degree of malignancy of each of the circulating tumor cells, characterized by the following steps: (a) bringing an epithelial cell-binding component, which specifically binds to a marker molecule expressed on epithelial cells and is fluorescently-labeled or luminescent enzyme-labeled, and a mesenchymal cell-binding component, which specifically binds to a marker molecule expressed on mesenchymal cells and is fluorescently-labeled or luminescent enzyme-labeled, into contact with a sample that possibly contains circulating tumor cells, (b) detecting a fluorescence signal or a luminescence signal of the epithelial cell-binding component and a fluorescence signal or a luminescence signal of the mesenchymal cell-binding component of each of the cells, and (c) determining the degree of epithelial-mesenchymal transition of circulating tumor cells based on the signal amount of the epithelial cell-binding component (E) and the signal amount of the mesenchymal cell-binding component (M).

Apparatus for detecting the degree of malignancy of each of circulating tumor cells

The present invention provides an apparatus for analyzing particles in a solution including a unit configured to place a flow cell having a flow path for flowing a sample solution containing the particles; a unit configured to illuminate the sample solution flowing through the flow path of the flow cell; a photodetector that detects a scattered light and/or fluorescence generated from the particles in the sample solution; and a unit configured to analyze the particles based on their signal intensities detected by the photodetector, wherein the flow cell has the flow path formed in a substrate, a reflection plane is formed on the side surface of the flow path, the reflection plane leads the lights generated in the flow path of the flow cell and advancing in the substrate in-plane direction to a specified region of the surface of the flow cell, and the photodetector detects the light exiting from the specified region to the outside.

Disposable chip-type flow cell and flow cytometer using same

The object of the present invention is to provide (1) a cell sorter, (2) a flow cytometer capable of detecting sideward scattered light, (3) a method for accurately measuring cell concentration, (4) a method for multicolor staining analysis without a fluorescence correction, and the like, which satisfy requirements that carry-over and cross contamination of samples do not occur. The object can be solved by an apparatus for separating particles comprising:

Disposable chip-type flow cell and cell sorter using the same

A method for sorting a particle of interest in a solution includes storing a sample solution in a sample solution reservoir and applying a constant pressure to the sample solution; illuminating the sample solution flowing in an illumination region of the sample solution flow path upstream of a branched flow path region, and detecting the particle of interest contained in the sample solution; and upon detecting the particle of interest, opening an electromagnetic valve or electromagnetic valves that are disposed between an air pressure pump and the branched flow path region of the sample solution flow path at a timing that the detected particle of interest reaches the branched flow path region such that a pulse pressure is applied to the branched flow path region to separate the detected particle of interest from the sample solution flow path.

Method for sorting cell particle in solution

A method for sorting a cell particle in a solution includes steps of preparing an apparatus having a sample solution reservoir formed on a substrate, a main flow path formed in the substrate, a first branched flow path and a second branched flow path, and a downstream flow path; storing a sample solution; applying a constant gas pressure to the sample solution, illuminating the sample solution; determining calculating a delayed timing; and applying a pushing pressure.

Conventional CTC detection methods have been problematic in that 1) there is no technique for automatically determining and counting live CTCs in a brief period of time, 2) no process has been developed for detecting, counting, and thereafter collecting and culturing live CTCs, and 3) there exists no flow cytometer that is contamination free and is capable of measuring an entire sample. Provided is a CTC detection method which comprises a pre-treatment step for concentrating and fluorescence staining CTCs, and a step for identifying and counting CTCs. The pre-treatment step includes attaching magnetic beads to EpCAM antibodies expressed by epithelial cell-derived CTCs and concentrating the CTCs through the use of a magnet, fluorescently labeling an epithelia cell surface marker of the CTCs through the use of EpCAM antibodies or 5E11 antibodies, and performing two types of nuclear staining, one being cell membrane-permeable and the other being cell membrane-impermeable. The identifying and counting step includes evaluating the respective absolute concentrations of live and dead CTCs in a volume of blood by automatically identifying CTCs by the ratio of a plurality of fluorescence signal intensities using a flow cytometer, and differentiating between and counting the live CTCs and the dead CTCs. In the cytometer, an entire liquid-feeding system that includes a flow cell can be replaced for each sample, and the total amount of a liquid sample can be measured.

Method for detecting low concentrations of specific cell from high concentrations of cell populations, and method for collecting and analyzing detected cell

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Tokyo, Japan

Kazuo Takeda