Riken Corporation

Ajinomoto Co., Ltd.

Other

Kazuo Shinozaki

Chieko Ohsumi

Teruaki Taji

Masatomo Kobayashi

Motoaki Seki

Satoshi Iuchi

Miki Fujita

Minami Matsui

Takanari Ichikawa

Miki Nakazawa

Taishi Umezawa

The present invention enables production of a plant resistant to environmental stresses including drought stress. The invention increases stress resistance to a plant by increasing raffinose content in the plant.

Method for increasing stress-resistance to a plant

A gene causative of a phenotype is easily specified and thus gene functions are comprehensively analyzed. A method for analyzing a gene function includes (a) infecting a plant group with a full-length cDNA library containing an expression regulatory sequence to introduce the cDNA into the plants; (b) selecting a plant complying with desired selection requirements from the plants into which the cDNA has been introduced; (c) isolating the cDNA from the selected plant; and (d) re-introducing the thus isolated cDNA into a plant of the same species as the plant into which the cDNA has been introduced, to reconfirm a phenotype according to the selection requirements.

Plant system for comprehensive gene function analysis with the use of full-length cDNA

A DNA encoding a neoxanthin cleavage enzyme used for improving stress tolerance in a plant, a method for increasing stress tolerance in a plant by introducing the DNA into the plant, and a transgenic plant into which a neoxanthin cleavage enzyme gene is introduced, are provided. A DNA used for reducing stress tolerance in a plant, a method for decreasing stress tolerance in a plant by introducing the DNA into the plant, and a transgenic plant into which the DNA is introduced, are also provided. The present invention enables creating a plant in which stress tolerance has been increased or decreased.

Transgenic plants carrying neoxanthin cleavage enzyme gene

The present invention provides a method of regulating expression of a gene by (a) preparing a recombinant plant cell line, plant tissue or plant with an expression vector having an abiotic environmental stress-responsive promoter of SEQ ID NO: 27 and the gene downstream thereof; and (b) culturing and cultivating the recombinant plant cell, plant tissue or plant under an abiotic environmental stress, wherein the promoter regulates the expression of the gene under the abiotic environmental stress.

Environmental stress-responsive promoter and a gene encoding environmental stress-responsive transcriptional factor

A stress-tolerant transgenic plant comprising an exogenously-introduced polynucleotide encoding a protein kinase which activates stress-responsive transcription factors. The polynucleotide may encode a protein kinase which comprises SEQ ID NO: 2 (SRK-2C) or a sequence having a significant degree of similarity with SEQ ID NO: 2. The transgenic plant has increased stress-tolerance to environmental stresses, such as to dehydration, osmotic stress or low temperature stress, compared to a similar non-transgenic plant.

Plant having tolerance to environmental stress

Method for providing a property of stress-resistance

The present invention enables production of a plant resistant to environmental stresses including drought and high salt concentration. The invention increases stress resistance to a plant by increasing galactinol content in the plant.

Growing community of inventors

Ibaraki, Japan

Kazuo Shinozaki