Ajinomoto Co., Ltd.

Iatron Laboratories Inc.

Kazuo Nakanishi

Natalia Pavlovna Zakataeva

Vladimir Veniaminovich Aleshin

Irina Lyvovna Tokhmakova

Vitaliy Arkadievich Livshits

Petr Vladimirovich Troshin

Hiroyuki Kojima

Yoshimi Kikuchi

Hiroshi Matsui

Tomoko Suzuki

Hisashi Yasueda

Yasushi Nishimura

Masao Motoki

Junichiro Kojima

Kazuo Nagase

Hisao Ito

Osamu Kurahashi

Yoshio Kawahara

Yuta Nakai

Kazuo Ogawa

Yuri Miyata

Setsuko Baba

In a method for producing a target substance utilizing a microorganism comprising culturing the microorganism in a medium to produce and cause accumulation of the target substance in the medium and collecting the target substance, there is used, as the microorganism, a mutant strain or a genetic recombinant strain constructed from a parent strain of the microorganism having a respiratory chain pathway of high energy efficiency and a respiratory chain pathway of low energy efficiency as respiratory chain pathways, and having either one or both of the following characteristics:

Method for producing substance utilizing microorganism

Anbacterium having dihydrodipicolinate synthase and aspartokinase, both of which are desensitized to feedback inhibition by L-lysine. The intracellular activity of dihydrodipicolinate reductase in this bacterium can also be enhanced. Furthermore, a diaminopimelate dehydrogenase gene can be introduced into this bacterium, or intracellular activities of tetrahydrodipicolinate succinylase and succinyl diaminopimelate deacylase can be enhanced. Finally, the intracellular activities of aspartate-semialdehyde dehydrogenase or phosphoenolpyruvate carboxylase can be enhanced in this bacterium. The bacterium can be cultured in a suitable medium to produce and accumulate L-lysine in culture, and the L-lysine is collected from the culture.

Method for producing L-lysine

Anbacterium (1) which harbors dihydrodipicolinate synthase of which feedback inhibition by L-lysine is desensitized and aspartokinase of which feedback inhibition by L-lysine is desensitized, (2) in which intracellular activity of dihydrodipicolinate reductase is enhanced, and (3) in which a diaminopimelate dehydrogenase gene is introduced or intracellular activities of tetrahydrodipicolinate succinylase and succinyl diaminopimelate deacylase are enhanced, wherein intracellular activity of aspartate-semialdehyde dehydrogenase or phosphoenolpyruvate carboxylase is enhanced, is cultured in a suitable medium to produce and accumulate L-lysine in culture, and the L-lysine is collected from the culture.

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A bacterium belonging to the genuswhich has an ability to produce an L-amino acid, wherein the ability to produce the L-amino acid is increased by increasing expression of the yfiK gene is described. A method for producing the L-amino acid using the bacterium is also described.

A bacterium belonging to the genuswhich has an ability to produce an L-amino acid, wherein the ability to produce the L-amino acid is increased by increasing expression of the yggA gene is described. A method for producing the L-amino acid using the bacterium is also described.

A bacterium belonging to the genuswhich has an ability to produce an L-amino acid, wherein the ability to produce the L-amino acid is increased by increasing expression of an L-amino acid excretion protein is described. A method for producing the L-amino acid using the bacterium is also described.

A bacterium belonging to the genusand having an ability to produce an L-amino acid, wherein the ability to produce the L-amino acid is increased by increasing an expression amount of an L-amino acid excretion protein, and a method for producing the L-amino acid using the bacterium.

Eschericha bacteria overexpressing the yahn gene for feedback-insensitive amino acid production

A DNA encoding aspartokinase III derived from bacteria of the genus Escherichia and having mutation by which feedback inhibition with L-lysine is released is introduced into cells to form transformant bacteria of the genus Escherichia. These bacteria are incubated in an appropriate culture medium. An L-amino acid is produced and accumulated in the culture, and collected from this culture.

Process for producing L-amino acid through fermentation

The present invention relates to a DNA fragment having a gene derived from fish which codes for a polypeptide possessing transglutaminase activity, a recombinant plasmid comprising a fish-derived DNA fragment which codes for a transglutaminase, a transformant into which a recombinant plasmid comprising a fish-derived DNA fragment which codes for a transglutaminase is introduced, and a method for the production of a transglutaminase, comprising culturing a transformant containing a fish-derived DNA fragment which codes for a transglutaminase.

Gene encoding transglutaminase derived from fish

In determining blood coagulation factor XIII in plasma, the activity of XIIIa, an activated form of the factor XIII, is measured by a method using casein and a fluorescent cadaverine derivative as the substrate and also featuring use of molecular sieve chromatography for separating a cadaverine derivative reacted with casein and an unreacted compound.

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Yokohama, Japan

Kazuo Nakanishi