The United States of America As Represented by the Department of Health

Kazunori Mine

Kenneth Alan Jacobson

Kenneth L Kirk

Markku I Linnoila

Thomas Miller

Primary and secondary amines in biological fluids are selectively derivitized by reaction with esters of N-hydroxysuccinimide or N-hydroxysulfosuccinimide to form an N-acylated derivative. Those N-acylated derivatives may then be extracted into a polar organic phase and extracted. Non-electroactive amines may be made suitable for coulometric analysis by selecting the ester used so as to attach an electroactive group to the amine. For example, histamine may be reacted with the Bolton-Hunter reagent to form a suitable derivative in high yield.

Growing community of inventors

Rockville, MD, United States of America

Kazunori Mine