Research Development Corporation of Japan

Cellseed, Inc.

Other

Transparent Inc.

Kao Corporation

Dai Nippon Printing Co., Ltd.

Japan Science and Technology Agency

Nippon Kayaku Kabushiki Kaisha

Japan Science and Technology Corporation

Nippon Oil Fats Co., Ltd.

Tokyo Women's Medical University

Toudai Tlo, Ltd.

Nanocarrier Co., Ltd.

K. K. Vayu

The University of Tokyo

Kazunori Kataoka

Yasuhisa Sakurai

Masayuki Yokoyama

Yukio Nagasaki

Masao Kato

Masayuki Yamato

Noriko Yamada

Teruo Okano

Yasuhiro Horiike

Nobuhiro Nishiyama

Shohei Inoue

Hidenori Otsuka

Akihiko Kikuchi

Kazuya Okamoto

Tatsuya Shimizu

Kohji Nishida

Kanjiro Miyata

Yoshishige Murata

Masatoshi Kuroda

Yasutaka Anraku

Shigeto Fukushima

Yoshikatsu Akiyama

Keiji Itaka

Akihiro Kishimura

Takayuki Amiya

Shigeru Kitano

Hideko Kanazawa

Tsuyoshi Miyazaki

Horacio Cabral

Akira Mamada

Michihiro Iijima

Kazuhisa Shimizu

Soichiro Okazaki

Teruo Nakamura

Nobuyuki Ohsako

Hidekazu Sekine

Yuichi Yamasaki

Makoto Oba

Isao Ishikawa

Yukio Hasegawa

Tomoya Suma

Kimihiro Yoshizako

Takamasa Onuki

Hiroko Mashiba

Yoshiyuki Koyama

Sayan Chuanoi

Teiji Tsuruta

Masato Kanzaki

Ryuji Uehara

Katsutoshi Kobayashi

Chieko Tsuchiya

Kazunori Waki

Daijiro Shiino

Wenjie Wang

Megumi Machida

Tomoko Akutsu

Hotaka Ito

Ken Suzuki

Takahisa Hayashida

Ryosuke Kodaka

Ung-il Chung

Shinsuke Ohba

Masateru Hasegawa

Tinatsu Kohno

Naoki Bamboo-House 203 Kanayama

Atsushi Gransifort-Nakamozu 3-1413 Harada

An improved process for producing a regenerated corneal endothelial cell sheet, comprising the steps of allowing corneal endothelial cells collected from a tissue to be cultivated on a cell culture support having its surface covered with a polymer of which the hydrating force varies in a temperature range of 0-80&#xb0; C., and after the culture, () adjusting the temperature of the culture solution to the temperature at which the polymer on the substrate surface is hydrated, () bringing the cultured corneal endothelial cell sheet into close contact with a carrier, and () detaching the sheet together with the carrier. The regenerated corneal endothelial cell sheet obtained by the process will adhere very well to living tissues.

Regenerated corneal endothelial cell sheets, processes for producing the same, and methods of using the same

The problem to be solved by the present invention is to provide a cultured cell sheet having a superior tissue adhesiveness and an excellent flexibility. The problems can be solved by producing the cultured cell sheet in which cells are cultured in the presence of a surfactant protein or a cross-linking inhibitor on a surface of a cell culture support that is coated with a temperature responsive polymer having an upper or lower critical solution temperature ranging from 0&#xb0; C. to 80&#xb0; C. in water, including steps of: adjusting the temperature of the culture medium to a temperature below the lower critical solution temperature or above the upper critical solution temperature; and detaching the cultured cell sheet.

Cultured cell sheet, production method, and tissue repair method using thereof

Provided is a method for easily and efficiently producing encapsulated substance vesicles wherein a substance is encapsulated in the cavity of vesicles obtained by polymer self-assembly. Empty vesicles that have membranes comprising a first polymer that is a block copolymer with uncharged hydrophilic segments and a first kind of charged segments and a second polymer with a second kind of charged segments that carry a charge that is the opposite of said first kind of charged segments as well as spaces that are enclosed by said membranes are mixed in an aqueous medium with the substance that is to be encapsulated in the spaces.

Substance-encapsulating vesicle and process for producing the same

This invention provides a cell pattern recovery tool comprising a base material layer having a surface subjected to easy adhesion treatment, a temperature responsive polymer layer that is provided on the base material layer and has a surface subjected to silane treatment, and a cell adhesion inhibiting material layer provided on the temperature responsive polymer layer. According to the present invention, a cell pattern can be rapidly recovered while maintaining the cell pattern stably and reliably under minimally invasive conditions for the cells.

Cell pattern recovery tool

A cultured periodontal membrane cell sheet, produced through a process comprising culturing cells on a cell culture support composed of a base material having its surface coated with a temperature-responsive polymer of 0&ring;C to 80&ring;C upper limit or lower limit critical dissolution temperature in water under specified conditions, (1) regulating the temperature of culture fluid to temperature&#x2267;the upper limit critical dissolution temperature or&#x2266;the lower limit critical dissolution temperature, (2) causing a cultured periodontal membrane cell sheet resulting from the culturing to adhere to a carrier, and (3) detaching the sheet together with the carrier. This cultured periodontal membrane cell sheet has a syndesmotic microstructure, exhibiting extremely high bioadherence to the surface of dental root, and realizes high-density transplant of target cells and positive reconstruction of periodontal tissue. Moreover, through laminating of cell sheets to be transplanted and donating of three-dimensional polarity, highly efficient reconstruction of attachment apparatus can be accomplished, and clinical application thereof to moderate periodontitis, severe periodontitis, gingival recession, etc. is highly promising.

Cultured periodontal ligament cell sheet, process for producing the same and method of use thereof

An anterior ocular segment related cell sheet or three-dimensional structure that have only a few structural defects as they have been recovered retaining the intercellular desmosome structure and the basement membrane-like protein between cell and substrate. The anterior ocular segment related cell sheet or three-dimensional structure is produced by a process comprising the steps of cultivating cells on a cell culture support comprising a substrate having its surface covered with a temperature responsive polymer having an upper or lower critical dissolution temperature of 0-80&#xb0; C. with respect to water, optionally stratifying the layer of cultured cells by the usual method, and thereafter,

Anterior ocular segment related cell sheets, three-dimensional structures, and processes for producing the same

Facing no ethical obstacle and easily to be isolated, multipotent concretely mesenchymal stem cells (MSCs) are one of the most powerful tools in reconstructive medicine. Here the inventors introduced 3D multicelluar spheroids culture construction based on photolithography and micropatterning techniques to improve multipotent differentiation efficiency of MSCs to adult cells. This invention, the 3D spheroid cultured construction for MSCs, leads to great improve of the differentiation efficiency. This invention relates to a cultured cell construct comprising a support; at least one island on the support; a hydrophilic and cytophobic substance applied on the surface of said support so as to encircle the island; and a spheroid which is derived from MSCs, said spheroid being in contact with the island.

Cultured cell construction containing spheroids of mesenchymal stem cells and utilization thereof

Parenchymal cells are cultivated on cultivated endothelial cells or cultivated fibroblasts which have been separated by a surface of a specific hydrophilic polymer, and which have been patterned. A culture which contains thus formed patterned spheroids of cultivated parenchymal cells is thereby provided by this invention. This culture maintains a function which is specific to the parenchymal cells over a long period of time.

Cultured cell construct which contains spheroids of cultured animal cells and the use thereof

The amount of drug consumption caused by a specific drug-metabolizing enzyme and/or the amount of the resulting metabolite(s) is measured by chromatography with an aqueous mobile phase under temperature control using a packing material whose surface is coated with a polymer having a hydration force varying in the temperature range of 0&#xb0; C. to 80&#xb0; C. This system enables proper evaluation of drug-metabolizing capacity through simple means without adversely affecting the environment.

High performance liquid chromatography with an aqueous mobile phase for analysis of drug and its metabolite

Cultured cell construct containing spheroids of cultured animal cells and utilization thereof

Provided is a complex comprising cisplatin encapsulated therein in a form in which a chlorine ion thereof is ligand-exchanged with a carboxyl anion of a block copolymer comprising poly(ethylene glycol)-poly(glutamic acid). This complex can be a medicinal preparation to which a novel dosage form decreased in toxicity can be applied.

Polymeric micelle containing cisplatin enclosed therein and use thereof

A temperature-responsive polymer and polymer material which has ester bond(s) and/or acid amide bond(s) respectively at one or more sites in the side chain and can be arbitrarily controlled by varying the side chain is provided.

Temperature-responsive polymer compound and process for producing the same

Polyoxyethylene derivatives having optionally protected amino group at one end and process for producing the same

Modified polymers containing a poly(2-hydroxyethyl(meth)acrylate) chain as the hydrophilic polymer segment in which a hydrophobic polymer chain or a lipid residue of a sterol is bound to either end of the poly(2-hydroxyethyl(meth)acrylate) chain through a covalent bond or in which the poly(2-hydroxyethyl(meth)acrylate) chain is grafted onto the backbone chain at either end thereof. These modified polymers are excellent in compatibility with liquid or a living body, thus being advantageously usable particularly in medical fields.

Modified polymers containing poly(2-hydroxyethyl(meth)acrylate segment in the molecule

This invention provides a heterotelechelic oligomer or polymer which is represented by the following formula: ##STR1## wherein A denotes a sugar residue, L denotes a linkage group represented by the following formula ##STR2## wherein R.sup.1 and R.sup.2 independently denote lower alkyl, aralkyl or aryl,

Polyoxyethylene having a sugar on one end and a different functional

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Ichikawa, Japan

Kazunori Kataoka