National Institute of Advanced Industrial Science and Technology

Chugai Seiyaku Kabushiki Kaisha

Kazunari Taira

Michinori Kohara

Renu Wadhwa

Sunil Kaul

Kyoko Kohara

Yoshio Kato

Makoto Miyagishi

Shiori Koseki

Jun Ohkawa

Junichi Matsuzaki

Yasuomi Takagi

Hiroshi Ohmori

Masumi Taki

The present invention provides a simple method for producing a dumbbell-shaped DNA. A method for producing a dumbbell-shaped DNA, wherein each of sense and antisense strands is connected at both the 5' and 3&#x2032; ends of a linear-shaped double stranded DNA by a single stranded DNA of loop structure, comprising the steps of;

Efficient process for producing dumbbell DNA

The present invention relates to cancer therapies using an antibody that binds to mortalin 2 and a functional nucleic acid. Mortalin expression was found to be upregulated in immortalized cells and tumor tissues. Immortalized human cells highly expressing mortalin showed anchorage-independent growth. When the K antibody, which is a specific anti-mortalin antibody, was injected into a tumor of a nude mouse, tumor growth was suppressed or the tumor shrank compared with the case of a control. In accordance with the present invention, the use of a specific anti-mortalin antibody (K antibody) for tumor therapies and the use of such antibody as a carrier molecule for transportation of immunotoxicin and the like into cells are provided. It has been shown that mortalin can be a target for cancer therapies. In accordance with the present invention, a novel and effective anticancer agent is provided. In addition, an anti-mortalin antibody that is internalized by cells is developed. Thus, various applications using such antibody are provided.

Use of anti-mortalin 2 antibody and functional nucleic acid for cancer therapies

Expression systems for functional nucleic acid expression

This invention provides a vector comprising cDNA encoding an RNA viral gene, characterized in that the vector is constructed such that both termini of the RNA viral gene can be transcribed precisely and uniformly. This vector is useful to elucidate the mechanisms of RNA virus replication and of the development of RNA virus infections, and to develop therapeutic agents and means of treatment, and so on.

Vector expressing the full-length gene of RNA virus and use thereof

Growing community of inventors

Ibaraki, Japan

Kazunari Taira