The Green Cross Corporation

Kazumoto Hirabayashi

Haruhide Kawabe

Tadakazu Suyama

Hirofumi Arimura

Masayuki Nishida

Ken Okabayashi

Masami Miura

Hideyuki Oi

Hazime Horii

Miho Shimizu

A plasmid for a yeast host, the plasmid comprising in sequence: (1) a yeast derived promoter, (2) an albumin-encoding region placed under control of the yeast-derived promoter, (3) a transcription terminator and (4) a sequence homologous to a part of the yeast host chromosomal sequence such that the plasmid is capable of being integrated into the yeast host cell chromosome and wherein the plasmid is incapable of autonomous replication in yeast cells; a yeast host transformed with the above plasmid; a method of producing a yeast transformant which comprises transforming a yeast host by integrating at least two different plasmids each having a sequence homologous to a part of the host yeast chromosomal sequence one at a time into the chromosome of the yeast host cell; and a method of producing albumin which comprises cultivating the above transformant and recovering the thus-produced albumin.

Albumin gene-containing plasmid, transformant carrying same, production

A recombinant plasmid wherein at least two DNA fragments containing a Baker gene and a Charlie gene of the yeast 2 .mu.m circular plasmid are incorporated into a plasmid containing genes encoding a physiologically active substance is disclosed. Also, a strain transformed by the recombinant plasmid is disclosed.

Recombinant plasmid and microbial strain transformed by said plasmid

Growing community of inventors

Osaka, Japan

Kazumoto Hirabayashi