The United States of America, As Represented by the Secretary, Department of Health and Human Services

The United States of America As Represented by the Secretary of

Kyoto University

Suntory Limited

The United States of America

Kazumitsu Ueda

Ira H Pastan

Michael M Gottesman

Shinya Yamanaka

Norio Nakatsuji

Yuji Shibano

Motonari Uesugi

Masato Nakagawa

Hirofumi Suemori

Eihachiro Kawase

Tohru Komano

Glenn Merlino

Nao Hirata

Hanan Galski

Asako Murata

Young-Tae Chang

Kaori Yamauchi

Yuto Fujibayashi

A method for sorting pluripotent cells using a compound which is eliminated from the pluripotent cells through the MDR1 transporter.

Method for sorting of pluripotent cells

The present invention provides for vectors carrying a cDNA containing the entire coding region of the human multidrug resistance gene (MDR1) and for a method for introducing MDR1 cDNA into cells thereby inducing a multidrug resistant phenotype.

Methods of inducing multidrug resistance using human MDR1 cDNA

Vectors carrying MDR1 cDNA which confer multidrug resistance on

Transgenic animals expressing a multidrug resistance cDNA

A yeast host which can express P-glycoprotein, i.e., the product of MDR-related gene, in the cell membrane in the same state as observed in multidrug resistant cells produced by connecting the MDR-related gene which carries multidrug resistance to a yeast expression vector and transforming the yeast with said recombinant vector; a cell membrane fraction containing a substantial amount of P-glycoprotein produced by said yeast and a process for the preparation thereof; and a recombinant vector for expressing the MDR-related gene in a yeast host.

Expression of MDR-related gene in yeast cell

Growing community of inventors

Kyoto, Japan

Kazumitsu Ueda