The Green Cross Corporation

Ishihara Sangyo Kaisha, Ltd.

Taisho Pharmaceutical Co., Ltd.

Yoshitomi Pharmaceutical Industries, Ltd.

Tanabe Seiyaku Company, Limited

Kaken Pharmaceutical Company, Ltd.

Mitsubishi Pharma Corporation

Yoshitomi Pharmaceutical

The Green Cross Company

Kazumasa Yokoyama

Chikara Fukaya

Tadakazu Suyama

Taizo Ono

Yoshio Tsuda

Yoshio Arakawa

Youichiro Naito

Yoshihisa Inoue

Masahiro Watanabe

Takao Ohmura

Ryoichiro Murashima

Akinori Sumi

Tsunetaka Nakajima

Hiroyuki Okamoto

Masakazu Iwai

Takahiro Haga

Toru Koyanagi

Yutaka Mizushima

Nobutoshi Yamada

Hideo Sugi

Nobuo Kondo

Shunichi Abe

Wataru Ohtani

Munehiro Noda

Kouichi Yamanouchi

Hideto Kodaira

Tadaaki Inoue

Kirby V Scherer, Jr

Kaeko Kamide

Koichi Yamanouchi

Kanemichi Okano

Kazuya Takeshima

Yoshihito Nawa

Kazuo Takechi

Ryozo Watanabe

Masahiro Kikuchi

Atsuyuki Ashimori

Toshiaki Akira

Tsunekazu Fukushima

Naoto Furuhata

Takae Kido

Kazuhiro Oohara

Syoichi Ishikawa

Masahide Kondo

Shigeo Ii

Yoshiro Iga

Yoshio Kagitani

Katsuhiro Uchida

Masanori Sugiura

Masao Tohno

Susumu Otomo

Hironaka Aihara

Yusei Shiraga

Nobuo Kondoh

Toru Iwao

Kenichi Hirai

Nagatoshi Fujiwara

Mamoru Tabata

Hiroshi Fukui

Shigeo Tanaka

Noboru Yamada

Tadao Okamoto

Shun-ichi Abe

Kiichiro Nabeta

Hitoshi Yasuda

Koichi Yamauchi

Kenji Tanaka

Kaoru Kobayashi

Yahiro Uemura

Yasuo Ueda

Hiroshi Shibata

Yutaka Hirao

Masayuki Nishida

Motonori Hashimoto

Makoto Okumura

Yoshinobu Hashimoto

Haruhide Kawabe

Shinobu Kuwae

Kenmi Miyano

Takashi Imagawa

Ken Okabayashi

Akimasa Ohmizu

Tsutomu Mimura

Yasuhiro Kohama

Naoto Fuluhata

Koji Munechika

Kiyonoshin Ichikawa

Shinji Tomioka

Hirotoshi Fukutsuka

Shirou Komeda

Hajime Fukuyama

Satoshi Morimoto

Shigetoshi Sugio

Ryoichi Naito, Deceased

Kimie Naito

Yasunari Yamaura

Tomoshi Ooya

Yasushi Matsuoka

Shinichiro Hase

Katsuhiro Uriyu

The invention provides a process for purifying recombinant human serum albumin (rHSA) by heating a-culture medium containing rHSA and the rHSA-producing host cells, feeding said heated solution upwardly into a fluidized bed in which adsorbent particles are suspended to effect contacting with the adsorbent particles and then recovering the adsorbed fraction containing the rHSA, and a composition comprising rHSA which shows a A350/A280 ratio of below 0.015, when formulated into a 25% solution of said albumin.

Process for purifying recombinant human serum albumin

Disclosed is an infusion preparation for nutrient supply use. It comprises a sugar, amino acids, electrolytes and a fat emulsion. It has an excellent shelf life without causing precipitation, denaturation and the like in spite of the simultaneous presence of these components. Also disclosed is a container filled with infusion liquids comprising a first and a second compartment separated from each other by a separation means, wherein an infusion liquid containing a fat emulsion and a sugar is included in the first compartment and another infusion liquid containing amino acids and electrolytes is included in the second compartment. Further disclosed are an infusion preparation comprising a fat emulsion and a sugar, and an infusion preparation comprising amino acids and electrolytes. An infusion preparation containing a sugar, amino acids, electrolytes and a fat emulsion can be obtained easily and aseptically upon use, by simply removing a separation means attached to the container and mixing the two infusion liquids.

Infusion preparation

An object of the present invention is to provide an infusion preparation set (a container filled with infusion liquids) useful for preparation of an infusion liquid containing sugars, amino acids, electrolytes, a fat emulsion and vitamins. The present invention is constituted by the use of a container having two compartments which are separated from each other by a separation means, which contains an infusion liquid comprising a fat emulsion, sugars, fat-soluble vitamins and specified water-soluble vitamins in the first compartment and an infusion liquid comprising amino acids, electrolytes and other specified water-soluble vitamins in the second compartment. An infusion preparation containing sugars, amino acids, electrolytes, a fat emulsion and vitamins can be obtained easily and aseptically upon use, by simply removing a separation means and mixing the infusion liquids included in the first and second compartments. Further, the components of the infusion liquids included in each compartment have good stability.

Container filled with infusion liquids and infusion preparation

Alimentative infusion liquids for administration through peripheral vein

Human serum albumin obtained by gene manipulation techniques can be purified by a combination of specified steps in which a culture supernatant obtained from a human serum albumin-producing host is subjected to ultrafiltration, heat treatment, acid treatment and another ultrafiltration, followed by subsequent treatments with a cation exchanger, a hydrophobic chromatography carrier and an anion exchanger, and by salting-out to thereby obtain a pure form of human serum albumin which contains substantially no proteinous and polysaccharide contaminants, which is formulated into a pharmaceutical preparation. The thus obtained human serum albumin can further be purified by treating recombinant human serum albumin with a hydrophobic chromatography carrier at pH of 2 to 5 and a salt concentration of 0.4 to 1 and exposing the carrier to a pH of 6 to 8 and a salt concentration of 0.01 to 0.3 M, or treating the culture supernatant with boric acid or a salt thereof at pH 8 to 11 for 1 to 10 hours and recovering the supernatant. This process makes it possible to effeciently purify recombinant human serum albumin and to provide substantially pure human serum albumin which does not contain producer host-related substances and other contaminants and is sufficiently free from coloration.

Recombinant human serum albumin, process for producing the same and

Disclosed is an infusion preparation for nutrient supply use. It comprises a sugar, amino acids, electrolytes and a fat emulsion. It has an excellent shelf life without causing precipitation, denaturation and the like in spite of the simultaneous presence of these components. Also disclosed is a container filled with infusion liquids comprising a first and a second compartments separated from each other by a separation means, wherein an infusion liquid containing a fat emulsion and a sugar is included in the first compartment and another infusion liquid containing amino acids and electrolytes is included in the second compartment. Further disclosed are an infusion preparation comprising a fat emulsion and a sugar, and an infusion preparation comprising amino acids and electrolytes. An infusion preparation containing a sugar, amino acids, electrolytes and a fat emulsion can be obtained easily and aseptically upon use, by simply removing a separation means attached to the container and mixing the two infusion liquids.

The invention provides a process for purifying recombinant human serum albumin (rHSA) by heating a culture medium containing rHSA and the rHSA-producing host cello, feeding said heated solution upwardly into a fluidized bed in which adsorbent particles are suspended to effect contacting with the adsorbent particles and then recovering the adsorbed fraction containing the rHSA, and a composition comprising rHSA which shows a A35D/A280 ratio of below 0.015, when formulated into a 25% solution of said albumin.

Infusion preparation and two compartment container containing the

Infusion preparation comprising phospholipid

A methylotrophic and glucotrophic mutant strain capable of producing a heterologous protein and a method for producing a heterologous protein, comprising culture of the mutant strain. The mutant strain of the present invention can be grown in a medium containing both methanol and glucose, with the effect that the growth of the strain and production of a heterologous protein proceed at the same time. Accordingly, a heterologous protein can be produced in a large amount in a short time.

Mutant strain of Pichia pastoris which utilizes methanol in the presence

The orally administrable pharmaceutical compositions containing a slightly water-soluble drug, characterized by improved stability and improved absorption of the drug from digestive tract into blood. The use of the compositions of the present invention enables decrease of the dose amount of a slightly water-soluble drug, which eventually leads to alleviation of pains and side effects on the part of patients.

Pharmaceutical Composition containing slightly water-soluble drug

Liquid preparation of antithrombin-III and stabilizing method therefor

Growing community of inventors

Osaka, Japan

Kazumasa Yokoyama