Hamamatsu-Photonics K.k.

Goryo Chemical, Inc.

Kazumasa Hirawake

Mitsuharu Miwa

Takayuki Sato

Yoshiaki Tezuka

Takahiro Shikayama

Takahiro Murakoshi

Itsushi Minoura

A fluorescence observation device is a device for performing fluorescence observation of a sample piece cut out from a sample including: a tray on which the sample piece is placed; a light source unit which generates excitation light to irradiate the sample piece; a detection unit which detects fluorescence from the sample piece; and an image generation unit which generates a fluorescence image of the sample piece based on a detection signal from the detection unit and the tray includes a plurality of placement regions provided around a center region of the tray and also includes a marker portion indicating a cutting orientation of the sample piece with respect to the sample.

Fluorescence observation device

A fluorescent image acquisition system includes a light emitting device which emits excitation light during ON period and stop the emission of the excitation light during OFF period, an imaging device which outputs ON image data and OFF image data, an input device which sets a coefficient, an image processing unit which creates fluorescent image data and background image data, and an identification unit which identifies fluorescent pixels and non-fluorescent pixels. In order to create a superimposed image, the image processing unit calculates values obtained by summing pixel values of fluorescent pixels of the fluorescent image data and pixel values of fluorescent pixels of the background image data at a ratio of 1:1, as pixel values of the fluorescent pixels and calculates values obtained by multiplying at least pixel values of the non-fluorescent pixels of the background image by the coefficient, as pixel values of the non-fluorescent pixels.

Superimposed image creation apparatus and superimposed image creation method

A fluorescence viewing device is configured to take a difference between one of an image of a first frame and an image of a second frame output from an imaging device and the other of the first frame image and the second frame image stored in an image storage means, whereby clear observed images can be obtained without influence of background light. In the fluorescence viewing device, an exposure time of a fluorescence image acquisition period and an exposure time of a background image acquisition period are different from each other. Since ON/OFF durations of excitation light also become asymmetric according to the asymmetry of the exposure times, the device can reduce user's feeling of strangeness due to blinking of the excitation light.

Fluorescence viewing device and fluorescence viewing method

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Hamamatsu, Japan

Kazumasa Hirawake