Other

Suzuki Motor Corporation

Tokyo Chemical Industry Co., Ltd.

Kazuko Matsumoto

Jingli Yuan

Yoshihisa Kawamura

Shinichi Teshima

Kazuhiro Matsui

Katsunori Ikeda

Guilan Wang

Mingqian Tan

It is intended to provide novel labeling reagents characterized by having a group capable of binding to a substance to be labeled (for example, a biological substance, a physiologically active substance, etc.), easily forming a complex together with a rare earth ion, the complex being stable in an aqueous solution, and having a sufficient fluorescence intensity and a long fluorescence life time regardless of buffer types; complexes composed of the above labeling reagent with a rare earth ion; fluorescence labels containing the above complex; a fluorescence assay method using the above fluorescent label; etc. Namely, labeling reagents comprising a compound having a 2,2':6&#x2032;,2&#x2033;-tripyridine skeleton or a 2,6-dipyrazolopyridine skeleton and having a group capable of binding to a substance to be labeled (for example, a biological substance, a physiologically active substance, etc.) and a group capable of forming a complex together with a rare earth ion; complexes composed of the above labeling reagent with a rare earth ion; fluorescence labels containing the above complex; a fluorescence labeling method using the above complex as a label; and a fluorescence assay method using the above fluorescent label.

Fluorescent label compounds

Insitu assay of substance in biological sample using labeled probe

Assay of substance in biological sample using labeled probe

Disclosed are labeling reagents containing fluorescent compounds represented by the general formula ##STR1## where R is a group capable of combining with proteins, Ar is a conjugated double bond system, and n is a whole number. These labeling reagents have high fluorescence emission intensities, give high synthesis yields, permit both solid-phase measurements and liquid-phase measurements in immunoassays, and require less measuring steps.

Labeled reagents for use in immunoassays and fluorescent compounds and

Growing community of inventors

Kawasaki, Japan

Kazuko Matsumoto