Nisshinbo Industries, Inc.

Japan Science and Technology Agency

Waseda University

Kazuko Matsumoto

Osamu Suzuki

Gen Masuda

Namiko Shiohata

Hideo Tashiro

Takahiko Nojima

Shigeori Takenaka

Yasumitsu Kondoh

Nishioka Takuya

Miyabe Masahito

Shinji Sueda

Yoshinori Yamaguchi

A rare-earth fluorescent complex which forms a fluorescent complex with two or more rare-earth metals such as europium and terbium and can effectively be excited at a wavelength of 340 nm or longer; a fluorescent labeling agent comprising the rare-earth fluorescent complex; a method of fluorescent labeling in which the rare-earth fluorescent complex is used as a labeling agent; and a method of fluorometric analysis in which the fluorescent labeling agent is used. The rare-earth fluorescent complex is characterized by having a cyclic ligand comprising a 4-biphenyl-2,2':6&#x2032;,2&#x2033;-terpyridine skeleton and a 2,6-bis(3&#x2032;-aminomethyl-1&#x2032;-pyrazolyl)pyrazine skeleton bonded thereto.

Fluorescent labeling compound

A practically usable DNA detection method by a DNA hybridization method with the use of fluorescent resonance energy transfer wherein the background luminescence based on the luminescence of a donor and the direct excitation of an acceptor) affecting the sensitized luminescence of the acceptor is minimized so that a target DNA strand can be conveniently and very easily detected in a homogeneous solution. In this DNA detection method, a DNA is detected by the DNA hybridization method by using, as detection reagents, streptoavidin labeled with a fluorescent rare earth metal complex, a nucleic acid probe modified with biotin and a nucleic acid probe labeled with an organic cyanine pigment and taking advantage of florescent resonance energy transfer.

Method of detecting DNA by DNA hybridization method with the use of fluorescent resonance energy transfer

Method for separating and collecting nucleic acids

Detecting reagent for double-stranded nucleic acid and double-stranded nucleic acid detecting method

Using the chemiluminescent group-containing carbodiimide compound represented by the following formula or its quaternary ammonium salt as the label in the nucleic acid detection method or immunoassay, labeling can be made efficiently for a short time, a nucleic acid derived from nature can be labelled, and highly sensitive assay is enabled. ##STR1##

Chemiluminescent group-containing carbodiimide compound

Using the fluorescent group-containing carbodiimide compound represented-by the following formula (I) as the label and the like in the nucleic acid detection method, immunoassay, or chemiluminescence assay, labeling can be made efficiently for a short time, a nucleic acid derived from nature can be labelled, and highly sensitive assay is enabled.

Fluorescent group-containing carbodiimide compound

As a novel digoxigenin derivative which makes the labeling procedure simple, can be used to label a naturally-occurring nucleic acid, and enables highly sensitive assay, carbodiimide group-containing digoxigenin derivative represented by the following formula is used as the label in a nucleic acid detection method or immunoassay. ##STR1##

Carbodiimide group-containing digoxigenin derivatives

Growing community of inventors

Tokyo, Japan

Kazuko Matsumoto