Riken Corporation

Japan Science and Technology Agency

The University of Tokyo

Mochida Pharmaceutical Co., Ltd.

University of Tsukuba

National Institute of Agrobiological Sciences

Kazuki Saito

Kiyoshi Ohyama

Hikaru Seki

Toshiya Muranaka

Hiroshi Sudo

Satoru Sawai

Shigeyuki Yokoyama

Tadao Asami

Osamu Nureki

Mikako Shirouzu

Ryuichiro Ishitani

Hiroshi Ezura

Masaki Mori

Tohru Ariizumi

Masami Hirai

Yoshihito Shinozaki

Hiroko Iijima

Naoyuki Umemoto

Hideo Ogiso

Fumio Matsuda

Satoru Maeda

Masashi Hikosaka

Junji Kimbara

Miyako Kusano

Atsushi Fukushima

Takashi Osanai

Ayuko Kuwahara

Sudo Hiroshi

Muranaka Toshiya

Seki Hikaru

Ohyama Kiyoshi

Sawai Satoru

Provided are an enzyme involved in a glycyrrhizin biosynthetic system, a gene of the enzyme and use thereof in order to stably and continuously provide a large amount of glycyrrhizin. Glucuronosyltransferase with an activity of further transferring glucuronic acid to the hydroxy group at the 2-position of glucuronic acid in an oleanane-type triterpenoid monoglucuronide is identified to provide the transferase, a gene for the transferase and use thereof.

Glucuronosyltransferase, gene encoding same and use thereof

It was found that it is possible to construct a genome-edited plant in which no exogenous gene is incorporated in a genome by performing negative selection using morphological defects and the like as indices in a regenerated plant originated from a plant cell in which a gene that induces regeneration of the plant and a gene of the genome editing enzyme are introduced.

Genome-edited plant production method

Provided is a plant such as tomato, in which plant vigor has been increased and heat resistance has further been imparted. A plant such as tomato can be obtained, which has increased the stem diameter as well as the plant height and has become heat resistant by having a mutant Della protein, in which a leucine corresponding to the leucine at position 567 in SEQ ID NO: 2 in the amino acid sequence of the Della protein has been replaced by another amino acid, preferably phenylalanine.

Mutant plant

An object of the present invention is to construct a production system that enables efficient production of organic acids using blue-green algae, which are photosynthetic microorganisms, by utilizing carbon dioxide and thereby increasing an amount of organic acids produced. The present invention relates to blue-green algae overexpressing a clock protein gene and a method for producing organic acids by culturing the blue-green algae.

Method for producing plastic raw material from blue-green algae

Identification of a protein having an activity of oxidizing oleanane-type triterpene, and a gene encoding the protein, the protein and the gene, and use thereof are provided. For example, a protein having an activity of oxidizing oleanane-type triterpene obtained from a plant in the family Fabaceae, a gene encoding the protein and use thereof are provided. The protein is shown in, for example, SEQ ID NO: 4, 14 or 18, and the gene encoding the protein is shown in, for example, SEQ ID NO: 3, 13 or 17. A transformant into which the gene is introduced can be produced, and thereby a triterpene oxidase can be obtained.

[object Object]

Provided is a protein having an activity of oxidizing a dammarane-type triterpene, a gene encoding the protein, and a method of using the protein and the gene. The protein can be obtained from a plant belonging to the genus, which has an activity of oxidizing a dammarane-type triterpene. Also provided is a transformant producing a triterpene oxidase into which a gene encoding the triterpene oxidase is introduced.

A compound useful as a plant activator for activating an endogenous defense system of a plant to control disease damage is provided. A compound represented by the formula: (R)NH&#x2014;(CH)&#x2014;N(R)&#x2014;(CH)&#x2014;NH(R) (one of Rand Rrepresents a linear Calkanoyl group or alkenoyl group, the other represents hydrogen atom or a protective group of amino group; and Rrepresents hydrogen atom or a protective group of amino group).

Plant activator

The present invention relates to a crystal of a complex of an epidermal growth factor (EGF) and an epidermal growth factor receptor (EGFR), a crystal of a complex of EGFR and a substance regulating EGFR activity, structure coordinates of these crystals, a method for screening for the substance regulating EGFR activity, a method for designing the substance regulating EGFR activity, a method for designing an EGFR variant or an EGF variant, a method for producing an EGFR variant or an EGF variant and an EGF variant or an EGFR variant obtainable by such method, a method for designing an epitope using the structure coordinates of the EGF-EGFR complex, a method for producing an anti-EGFR antibody or an anti-EGF antibody and an antibody obtainable by such method, a polypeptide or a salt thereof comprising a region that forms an EGFR dimerization site, and the like.

EGR-EGFR complex

Growing community of inventors

Chiba, Japan

Kazuki Saito