Sysmex Corporation

National Center for Global Health and Medicine

Kazuki Nakabayashi

Yasuhiro Otomo

Hideki Takata

Kayo Shoji

Keiichiro Shohmi

Shigeki Abe

Koichi Yamagata

Tomokazu Yoshida

Masashi Mizokami

Youichi Takahama

Takayuki Takahata

Sachiyo Tada

Kazuhiko Takeda

Motonari Daito

Masahiro Nishida

Masaya Sugiyama

Noriko Kinoshita

Norio Ohmagari

Hiroyuki Shimizu

Disclosed is a method for assisting prediction of exacerbation of respiratory infection, comprising measuring a biomarker in a specimen collected from a subject suffering from a respiratory infection or a subject suspected of having a respiratory infection, wherein the biomarker is at least one selected from the group consisting of IFNλ3, CCL17, CXCL11, IP-10, IL-6 and CXCL9, and a measured value of the biomarker is used as an index to predict exacerbation of the respiratory infection.

Method for assisting prediction of exacerbation of respiratory infection, and device to assist in predicting exacerbation of respiratory infection

Methods of judging the lymph node metastasis of stomach cancer, apparatuses for judging the lymph node metastasis of stomach cancer and kits used therefor are disclosed.

Method of judging lymph node metastasis of stomach cancer

A treatment solution for preparing a sample solution for nucleic acid amplification reaction is described. The treatment solution comprises dimethyl sulfoxide and aqueous solvent. The sample solution prepared by treating a biological sample with the treatment solution is used in the amplification reaction of a nucleic acid, whereby the nucleic acid can be efficiently recovered from a biological sample, and the influence of an inhibitor during nucleic acid amplification can be effectively reduced.

Treatment solution for preparing sample solution for nucleic acid amplification reaction and method for detecting nucleic acid by using treatment solution

The present invention provides a novel marker capable of accurately diagnosing the lymph node metastasis of stomach cancer. An mRNA, or a fragment thereof, coding for at least one protein selected from TFF1, AGR2, PRSS8, MUC1, MUC4 and MUC17 can be useful as a lymph node metastasis marker.

Method for judging lymph node metastasis of stomach cancer and kit used therefor

The invention provides a method for judging whether cancer cells are present or not in a sample, comprising a obtaining step of obtaining values related to an expression level of a caner marker gene and a housekeeping gene; a first comparing step of comparing the value related to the expression level of the cancer marker gene with a first threshold value; a normalizing step of normalizing the value related to the expression level of the cancer marker gene based on the value related to the expression level of the housekeeping gene; a second comparing step of comparing the normalized value with a second threshold value; and a judging step of judging whether cancer cells are present or not in the sample based on comparison results obtained in the first and second comparing steps, as well as an apparatus for judging whether cancer cells are present or not.

Method and apparatus for judging the presence or absence of cancer cell

In detecting a nucleic acid by using the nucleic acid amplification method, it is intended to shorten the time required for the measurement so as to systematically and efficiently examine a gene, etc. To achieve this object, use is made of a method of directly amplifying a nucleic acid by treating a collected biological sample wherein a means of inhibiting the degradation activity of the nucleic acid is introduced during or after the step of homogenizing the biological sample and thus the nucleic acid is directly amplified without isolating/purifying the nucleic acid component from the biological sample. In this method, the means of inhibiting the degradation activity of the nucleic acid is introduced at an acidic pH value (more specifically, from pH 2.5 to pH 5) with the use of a salt interacting with a substance inhibiting the nucleic acid amplification reaction and/or the nucleic acid component so as to directly amplify the nucleic acid without isolating/purifying the nucleic acid component.

Method and system for detection of nucleic acids

Growing community of inventors

Kobe, Japan

Kazuki Nakabayashi