Sysmex Corporation

Osaka University

Nec Network and Sensor Systems, Ltd.

Kazuki Kishi

Masakazu Fukuda

Masaki Ishisaka

Takashi Ishikawa

Junyi Ding

Yuichi Yasuda

Kengo Gotoh

Noriaki Sumita

Masakatsu Morita

Yasutaka Kuroda

Shinzaburou Noguchi

Yasuto Naoi

Tomohiko Oohashi

Rieko Goto

Yoshiaki Sota

Ringing of a signal after passing through a low pass filter in an LINC type linear amplifier is remedied. A linear amplifying device includes the linear amplifier including the low pass filter for removing high-frequency components; and an origin avoiding circuit which receives an original input signal and is provided upstream of the linear amplifier. If the original input signal is sampled in the vicinity of an origin, the origin avoiding circuit modifies a sampled value so at to replace the sampled value with a fixed value, and supplies the modified input signal to the linear amplifier.

Linear amplifying device, input signal supplying method, and origin avoiding circuit used therein

A cell analysis apparatus includes an optical detection section that accommodates a flow of a measurement sample obtained from a biological sample and a pigment into a flow cell. A light source irradiates the measurement sample flowing in the flow cell and a fluorescence detector detects fluorescence from the measurement sample. A signal processing circuit acquires a value reflecting the height of a waveform of the signal and a value reflecting the length of a ridge line of the waveform of the signal, and a system control section distinguishes between an aggregating cell formed by aggregation of a plurality of cells and a non-aggregating cell, based on the value of the fluorescence signal obtained by the signal processing circuit.

Cell analysis apparatus and cell analysis method

A cell analysis apparatus that can accurately distinguish between an aggregating cell and a non-aggregating cell is provided. The cell analysis apparatus () includes: an optical detection section () for flowing a measurement sample obtained from a biological sample and a pigment into a flow cell (), irradiating the measurement sample flowing in the flow cell () with laser beam and detecting fluorescence from the measurement sample; a signal processing circuit () for acquiring, based on a fluorescence signal outputted from the detection section (), a value reflecting the height of a waveform of the signal and a value reflecting the length of a ridge line of the waveform of the signal; and a system control section () for distinguishing between an aggregating cell formed by aggregation of a plurality of cells and a non-aggregating cell, based on the value reflecting the height of the waveform of the fluorescence signal and the value reflecting the length of the ridge line of the waveform of the fluorescence signal obtained by the signal processing circuit ().

Disclosed is a method for examining prognosis of breast cancer including the steps of: (A) extracting RNA from a specimen collected from a subject, (B) preparing a determination sample using the extracted RNA, (C) determining the expression level of each gene in the specific gene groups using the obtained determination sample, (D) analyzing the expression level of the determined each gene, and (E) examining prognosis of breast cancer, based on the obtained analysis result are performed.

Method for examining prognosis of breast cancer

A method of activating an antigen is herein described. The method comprises providing an antigen activation solution and contacting the cell with the solution to activate the antigen. A method of detecting a cell fixed by a nonbridging fixation solution is also described. The method comprises providing an antigen activation solution, contacting the cell with the solution, immunostaining the cell, and detecting the stained cell. The solution used for these methods comprises an agent for breaking a hydrogen bond, and is also described herein.

Method for activating an antigen, method for detecting a cell, and solution for activating an antigen

Growing community of inventors

Tokyo, Japan

Kazuki Kishi