Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo

Hayashibara Co., Ltd.

Kazuhisa Mukai

Toshio Miyake

Michio Kubota

Shigeharu Fukuda

Hiroto Chaen

Tomoyuki Nishimoto

Hikaru Masahiro Watanabe

Keiji Tsusaki

Hikaru Watanabe

The object of the present invention is to provide a method and a process for producing 2-O-&#x3b1;-glucopyranosyl-L-ascorbic acid where 5-O-&#x3b1;-glucopyranosyl-L-ascorbic acid and 6-O-&#x3b1;-glucopyranosyl-L-ascorbic acid are not formed or formed in such a small amount that the formation of these can nor be detected. The present invention solves the above object by providing a process for producing 2-O-&#x3b1;-glucopyranosyl-L-ascorbic acid comprising the steps of allowing &#x3b1;-isomaltosyl glucosaccharide-forming enzyme together with or without cyclomaltodextrin glucanotransferase (EC 2.4.1.19) to act on a solution comprising L-ascorbic acid and, an &#x3b1;-glucosyl saccharide to form 2-O-&#x3b1;-glucopyranosyl-L-ascorbic acid and collecting the formed 2-O-&#x3b1;-glucopyranosyl-L-ascorbic acid.

Process for producing 2-O-alpha-D-glucopyranosyl-L-ascorbic acid

An object of the present invention is to provide an option of non-reducing saccharide by providing a novel non-reducing saccharide composed of glucose as constituents and to provide a novel enzyme forming the non-reducing saccharide, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the non-reducing saccharide, and uses thereof. The present invention solves the above object by providing a novel cyclic saccharide having a structure of cyclo{&#x3e;6)-&#x3b1;-D-glucopyranosyl-(1&#x3e;4)-&#x3b1;-D-glucopyranosyl-(1&#x3e;6)-&#x3b1;-D-glucopyranosyl-(1&#x3e;4)-&#x3b1;-D-glucopyranosyl-(1&#x3e;}, cyclic maltosylmaltose, novel cyclic maltosylmaltose-forming enzyme, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the cyclic maltosylmaltose or a saccharide composition comprising the same, and uses thereof.

Cyclic maltosylmaltose, cyclic maltosymaltose-forming enzyme, their preparation and uses

An object of the present invention is to provide an option of non-reducing saccharide by providing a novel non-reducing saccharide composed of glucose as constituents and to provide a novel enzyme forming the non-reducing saccharide, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the non-reducing saccharide, and uses thereof. The present invention solves the above object by providing a novel cyclic saccharide having a structure of cyclo{&#x2192;6)-&#x3b1;-D-glucopyranosyl-(1&#x2192;4)-&#x3b1;-D-glucopyranosyl-(1&#x2192;6)-&#x3b1;-D-glucopyranosyl-(1&#x2192;4)-&#x3b1;-D-glucopyranosyl-(1&#x2192;6}, cyclic maltosylmaltose, novel cyclic maltosylmaltose-forming enzyme, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the cyclic maltosylmaltose or a saccharide composition comprising the same, and uses thereof.

Cyclic maltosylmatose

A stable crystalline powdery saccharide having a crystallinity of 40% or more, less hygroscopicity, satisfactory fluidity, and beneficial handleability, which is obtainable from an aqueous solution, containing trehalose and a different saccharide(s) crystallizable in the presence of trehalose, by crystallizing the trehalose along with the different saccharide(s).

Crystalline powdery saccharide, its preparation and uses

Crystalline powdery saccsharide, its preparation and uses

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Okayama, Japan

Kazuhisa Mukai