Arkray, Inc.

Kazuhiro Ohmiya

Shinya Nakajima

Takashi Nakagawa

Tokuo Kasai

Kyouichi Ohshiro

Tomoko Hirayama

Kaori Ishizaki

Naoko Izui

Provided is an analysis method in which increase in a background can be prevented in a simple manner without cost. The analysis method of the present invention is carried out using a sample analysis toolincluding a development memberin which a developing solution supply portion, a sample supply portion, and a detection portionin which a substancethat specifically binds to an analytein a sample is immobilized are provided in this order from upstream to downstream along the flow of a developing solution. A sample solution is supplied to the sample supply portion, and a developing solution is supplied to the developing solution supply portionsimultaneously with the supply of the sample solution or prior to the supply of the sample solution. By the development of the developing solution in the development memberin the presence of a labeled specifically binding substance, the sample solution is introduced to the detection portion. In the detection portion, a complexof the immobilized specifically binding substance, the analyte, and the labeled specifically binding substanceis formed. The analyteis analyzed by detecting a labelin the complex

Method for analyzing, sample analysis tool, method for preventing flow of sample solution in undesired direction, and method for preventing increase in background

Provide is a sample analysis tool whose reactivity and reproducibility in analysis can be prevented from decreasing. The sample analysis toolof the present invention includes a development memberand a plastic base, and at least part of the development memberis in contact with the plastic base. The sample analysis toolfurther includes a hydrophilic component layer, and the hydrophilic component layeris formed on part or the whole of at least one of a surface of the plastic baseand a surface of the development member. It is particularly preferable that the hydrophilic component layercontains sucrose or N-methyl glucosamine. In the sample analysis toolof the present invention, since the hydrophilic component layeris formed on part or the whole of at least one of the surface of the plastic baseand the surface of the development member, it is possible to prevent the adhesion of a hydrophobic component(s) derived from the plastic base

Sample analysis tool, method for producing sample analysis tool, and method for inhibiting decrease in liquid permeability of development member

A reducing power analysis method for minimizing peak wavelength shift in a sample, comprising a reduction step of reducing a dye reagent containing a ferric compound and a cyanide at pH conditions of 2.4 or lower in the presence of a sample; and an optical measurement step of optically measuring a peak wavelength of a reduced form of the dye reagent obtained in the reduction step.

Reducing power analysis method and reducing power analysis reagent

A specimen supplying tool in which structure is simple, and a liquid specimen and air bubbles do not remain within a through-bore for introduction of a specimen, is provided. The specimen supplying tool includes a substrateand a through-boreprovided in the substrate. One opening of the through-boreis a specimen inflow port, and the other opening of the through-boreis a specimen outflow port. The specimen supplying tool further includes a projection portionprojecting from a part p of a marginal part of the specimen outflow port. A liquid specimen is introduced from the specimen inflow port to the through-bore. When the introduced liquid specimen is discharged from the specimen outflow port, and supplied to a specimen analyzing instrument, the liquid specimen is drawn to the projection portion, led by the projection portion, and supplied to the specimen analyzing instrument.

Specimen supplying tool and specimen analysing instrument using the same

The present invention provides a method of pretreating a specimen, which allows measurement according to an immunoassay to be carried out on a specimen from nasal secretion while preventing non-specific reactions. According to this method, the specimen from nasal secretion is treated with a protease beforehand and then an immunoassay is performed. As the protease, it is preferable to use semi-alkaline protease (EC 3.4.21.63). Furthermore, it is preferable that a substance to be pretreated by the pretreatment method according to the present invention is an influenza virus contained in the specimen from nasal secretion. The immunoassay preferably is an immunoagglutination assay. Examples of the immunoagglutination assay include a turbidimetric immunoassay, a latex turbidimetric immunoassay, and a latex agglutination assay that is performed on a slide glass.

Method of pretreating specimen and immunoassay using the same

An optical measurement apparatus is provided for conducting a test by efficiently reading color development of a reagent after reaction by optical measurement. To the above end, an optical measurement apparatus is provided which is to be used with at least one test instrument mounted to the apparatus. The test instrument includes a carrier provided with at least one reagent retaining portion which retains a reagent, and a sample is applied to the carrier. The optical measurement apparatus includes a reader for reading color development of the reagent retaining portion, and a controller for performing driving control of the reader and determination. The controller performs the determination by utilizing data obtained by a reading operation P to read the color development of the reagent performed after the lapse of a reaction completion period Tr-Trfrom the mounting of the test instrument, and the reaction completion period depends on the reagent.

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Kyoto, Japan

Kazuhiro Ohmiya