Medinet Co., Ltd.

Other

Kazuhiro Kakimi

Junichiro Futami

Ryuji Maekawa

Mie Nieda

Manami Isogai

Masato Shiraki

Manami c/o Medinet COLTD Isogai

Mie Medinet Co Ltd Nieda

The present invention provides the following: a method for efficiently producing a reagent for detecting an antibody that specifically binds with an insoluble antigen protein present in a liquid sample; a reagent for antibody detection produced by the production method; and a use of the antibody. In a step for solubilizing an antigen protein, it is possible to efficiently solubilize and recover the antigen protein by using a cationizing agent; therefore, when compared to conventional methods, it is possible to efficiently produce a reagent for detecting an antibody that has bound to multiple antigen protein molecules in a carrier.

Method for producing reagent for antibody detection and use thereof

The present invention provides dendritic cells capable of efficiently activating and/or proliferating &#x3b3;&#x3b4; T-cells in vivo and/or in vitro, pharmaceutical compositions comprising said dendritic cells, therapeutic methods and &#x3b3;&#x3b4; T-cell culture methods utilizing said dendritic cells. By pulsing immature dendritic cells derived from peripheral blood monocytes with a bisphosphonate-based bone metabolism improving drug to enable the cells to activate &#x3b3;&#x3b4; T-cells, and cultivating them with a cell subset containing &#x3b3;&#x3b4; T-cells, &#x3b3;&#x3b4; T-cells can be activated and/or proliferated. This allows for easy proliferation of &#x3b3;&#x3b4; T-cells without burdening a patient, leading to practical applications of immune cell therapies that utilize &#x3b3;&#x3b4; T-cells.

Dendritic cells pulsed with a bisphosphonate

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Tokyo, Japan

Kazuhiro Kakimi