Jsr Corporation

Kazuhiro Ikkyu

Hiroshi Kawai

Kouji Tamori

Yong Wang

Tetsuo Fukuta

Takayoshi Abe

Satoshi Nakamura

Tomonori Shiotani

Yusuke Okano

Satoshi Hyugaji

Masaki Momiyama

Yu Otani

Provided are polymer particles which can be used at a high flow rate when used as a filler for chromatography, that is, has excellent resistance flow rate appropriate for processing in large quantities, and also has a high binding capacity for target molecules such as proteins when an appropriate ligand is contained in the particles, and a method for producing the polymer particles; specifically, crosslinked polymer particles and a method for producing the crosslinked polymer particles, polysaccharide composite particles and a method for producing the polysaccharide composite particles, a filler for chromatography using the polymer particles, and an adsorbent for antibody purification. Disclosed are: A. a method for producing polysaccharide composite particles, the method including the following steps (1) to (3): (1) a step of preparing a polysaccharide solution, in which two or more kinds of polysaccharides are dissolved in an ionic liquid; (2) a step of preparing a droplet dispersion liquid of the polysaccharide solution, in which liquid droplets of the polysaccharide solution are dispersed in an organic solvent having low compatibility with the ionic liquid; and (3) a coagulation step in which a composite of the polysaccharides are coagulated to obtain the polysaccharide composite particles; and B. a method for producing a crosslinked polymer particle, the method including a step of allowing a polymer dissolved in an ionic liquid, to react with a crosslinking agent while the polymer is subjected to droplet dispersion in an organic solvent having low compatibility with the ionic liquid.

Method for producing polymer particles, and polymer particles

Provided is a filler for affinity chromatography having a high dynamic binding capacity for proteins and having excellent alkali resistance and storage stability. The filler for affinity chromatography of the present invention comprises a porous particle consisting of a copolymer of 40 parts to 99.5 parts by mass of (M-1) a methacryloyl group-containing vinyl monomer that contains a hydroxyl group and does not contain an epoxy group, 0.5 parts to 30 parts by mass of (M-2) an epoxy group-containing vinyl monomer, 0 parts to 59.5 parts by mass of (M-3) a methacryloyl group-containing vinyl monomer which is other than the monomers (M-1) and (M-2), and 0 parts to 25 parts by mass of (M-4) a vinyl monomer other than the monomers (M-1), (M-2) and (M-3) (with the proviso that the total amount of the contents of (M-1), (M-2), (M-3) and (M-4) is 100 parts by mass); ring-opened epoxy group obtainable by ring-opening of epoxy group that is contained in the copolymer; and ligand that is bound to the porous particle.

Filler for affinity chromatography

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Minato-ku, Japan

Kazuhiro Ikkyu