Sysmex Corporation

Kazuhiko Takeda

Shigeki Abe

Yasuhiro Otomo

Kazuki Nakabayashi

Tadahiro Kajita

Takahiro Yamagaito

Ayumi Asaeda

Takuya Koma

Mari Maruki

The present invention relates to a pretreatment method of a sample for detecting HBs antigen, which is a surface antigen of the hepatitis B virus, and a method for detecting HBs antigen utilizing the pretreatment method. The present invention also relates to a pretreatment reagent kit for detecting HBs antigen.

Pretreatment method of sample for detecting HBs antigen and use thereof

A treatment solution for preparing a sample solution for nucleic acid amplification reaction is described. The treatment solution comprises dimethyl sulfoxide and aqueous solvent. The sample solution prepared by treating a biological sample with the treatment solution is used in the amplification reaction of a nucleic acid, whereby the nucleic acid can be efficiently recovered from a biological sample, and the influence of an inhibitor during nucleic acid amplification can be effectively reduced.

Treatment solution for preparing sample solution for nucleic acid amplification reaction and method for detecting nucleic acid by using treatment solution

An anti-HBs monoclonal antibody is described herein. This antibody can bind to the following: (i) a wild type HBsAg; (ii) at least one mutant HBsAg selected from the group consisting of a first mutant HBsAg and a second mutant HBsAg; and (iii) at least one mutant HBsAg selected from the group consisting of a third mutant HBsAg and a fourth mutant HBsAg. The first mutant HBsAg has a mutation at position 120. The second mutant HBsAg has a mutation at position 141. The third mutant HBsAg has a substitution to a lysine at position 118. The fourth mutant HBsAg has only one mutation at position 144 and an amino acid at the position 144 is substituted by a glutamic acid.

Anti-HBs monoclonal antibody

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Yao, Japan

Kazuhiko Takeda