Kyowa Hakko Bio Co., Ltd.

Kyowa Hakko Kogyo Co., Ltd.

Kazuhiko Tabata

Akio Ozaki

Satoshi Koizumi

Tetsuo Endo

Shin-ichi Hashimoto

Katsutoshi Sasaki

Yoshiyuki Yonetani

Mikiro Hayashi

Hajime Ikeda

Shin-Ichi Hashimoto

Hiroshi Nagano

Makoto Yagasaki

Tetsuro Ujihara

Akihiro Senoo

Shoto Ohno

Aya Hadakubota

Aya Kubota

Tetsuo Nishimura

Shizuo Tsuchiya

Megumi Nagano

Aya Hada

According to the present invention, theanine can efficiently be produced without exogenously adding ethylamine and without accumulation or leftover of ethylamine as a byproduct, by using a microorganism having enhanced activity to produce ethylamine with acetaldehyde and alanine as substrates and having enhanced activity of &#x3b3;-glutamylmethylamide synthetase or glutaminase.

Method for producing theanine

According to the present invention, a microorganism belonging to the genushaving an 18S rRNA gene consisting of the base sequence represented by any of SEQ ID NOS: 1 to 5;sp. OH4 strain; a microorganism which is a mutant obtained from the above-mentioned microorganism as a parent strain and has a higher ability to produce DHA than the parent strain; orsp. LTR23 strain is provided. Also, a method for producing a DHA-containing composition, DHA and DHA alkyl ester by a fermentation process using the above-mentioned microorganisms is provided.

Microorganism producing docosahexaenoic acid and utilization thereof

Provided is a novel process for producing an L-amino acid using a microorganism belonging to the genus. According to the present invention, a process for producing an L-amino acid comprising; culturing a microorganism in which an activity of the protein of any of the following [1]-[3] is increased compared with that of the parent strain in a medium, producing and accumulating the L-amino acid in the medium, and recovering the L-amino acid from the medium:

Process for production of L-amino acid

Provided is an efficient process for producing L-glutamine or L-glutamic acid using a microorganism. L-glutamine or L-glutamic acid is produced by culturing in a medium a microorganism in which has an ability to produce L-glutamine or L-glutamic acid, and in which an ability to form a superhelical double-stranded DNA is decreased compared with that of the parent strain, producing and accumulating L-glutamine or L-glutamic acid in the medium, and recovering L-glutamine or L-glutamic acid from the medium.

Process for production of L-glutamine or L-glutamic acid

The present invention provides: (1) crystals of a dipeptide which do not substantially comprise a dipeptide comprising D-amino acid as a constituent or a polypeptide consisting of three or more amino acids; and (2) crystals of a dipeptide which do not substantially comprise a dipeptide comprising D-amino acid as a constituent, a polypeptide consisting of three or more amino acids, or an amino acid amide; and a process for producing the dipeptide crystals.

L-alanyl-L-glutamine crystal

The invention provides a microorganism having an ability to produce a protein having a dipeptide synthesizing activity and in which an activity of the protein to transport a dipeptide in a microbial cell to theoutside of the microbial cell is higher than that of a parental strain.

Method for producing dipeptide

The invention provides a process for producing glutathione or &#x3b3;-glutamylcysteine by culturing in a medium a microorganism with a higher activity of a protein having an activity to transport intracellular glutathione to the outside of cells, and a higher activity of a protein involved in glutathione or &#x3b3;-glutamylcysteine biosynthesis, compared with that of the parent strain, forming and accumulating glutathione or &#x3b3;-glutamylcysteine in the medium, and recovering the glutathione or &#x3b3;-glutamylcysteine from the culture.

Method for production of glutathione or gamma-glutamylcysteine

The present invention provides a method of improving efficiency of a fermentative production of an L-amino acid. To be specific, the present invention provides a process for producing a L-amino acid, comprising culturing a microorganism wherein the activity of the protein described in any one of (1) to (3) below is higher than that of the parent strain in a medium to produce the L-amino acid and accumulate the L-amino acid in the medium, and then collecting the L-amino acid from the medium:

Process for producing L-amino acid

This invention relates to a process for producing a sugar nucleotide, in which a) a culture broth of a microorganism capable of producing NTP from a nucleotide precursor, or a treated product of the culture broth, and b) a culture broth of a microorganism capable of producing a sugar nucleotide from a sugar and NTP, or a treated product of the culture broth, are used as enzyme sources; a process for producing a complex carbohydrate, in which the above-described a) and b) and c) a culture broth of a microorganism, an animal cell or an insect cell capable of producing a complex carbohydrate from a sugar nucleotide and a complex carbohydrate precursor, or a treated product of the culture broth, are used as enzyme sources; a process for producing a complex carbohydrate, in which a culture broth of a microorganism, an animal cell or an insect cell capable of producing a complex carbohydrate from a sugar nucleotide and a complex carbohydrate precursor, or a treated product of the culture broth, is as an enzyme source; and a process for producing N-acetylglucosamine-1-phosphate, in which a culture broth of a microorganism having strong galactokinase activity, or a treated product of the culture broth, is used as the enzyme source.

Processes for producing sugar nucleotides and complex carbohydrates

The present invention provides a microorganism belonging to the genusin which the activities of glutamine-synthetase adenylyltransferase (GlnE protein) and PII regulatory protein for glutamine synthetase are reduced or lost and which has the ability to form and accumulate L-glutamine or a substance biosynthesized utilizing nitrogen supplied by L-glutamine, and a process for producing L-glutamine or the substance biosynthesized utilizing nitrogen supplied by L-glutamine using the microorganism.

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The present invention provides a protein which catalyzes the synthesis of a dipeptide different from L-Ala-L-Ala, a process for producing the protein which catalyzes the synthesis of a dipeptide, a process for producing a dipeptide using the protein which catalyzes the synthesis of a dipeptide, and a process for producing the dipeptide using a culture of a microorganism producing the protein which catalyzes the synthesis of a dipeptide or the like as an enzyme source.

Process for producing dipeptides

The present invention provides a process for producing a dipeptide which comprises culturing in a medium a microorganism which has the ability to produce a protein having the activity to form the dipeptide from one or more kinds of amino acids and which has the ability to produce at least one of said one or more kinds of amino acids, allowing the dipeptide to form and accumulate in the medium, and recovering the dipeptide from the medium.

The present invention provides a process for economically producing N-acetylneuraminic acid without using expensive materials such as pyruvic acid and phosphoenolpyruvic acid. The process comprises: allowing (i) a culture of a microorganism having N-acetylneuraminic acid aldolase activity or N-acetylneuraminic acid synthetase activity, or a treated matter of the culture, (ii) a culture of a microorganism capable of producing pyruvic acid or a treated matter of the culture, or a culture of a microorganism capable of producing phosphoenolpyruvic acid or a treated matter of the culture, (iii) N-acetylmannosamine, and (iv) an energy source which is necessary for the formation of pyruvic acid or phosphoenolpyruvic acid to be present in an aqueous medium to form and accumulate N-acetylneuraminic acid in the aqueous medium; and recovering N-acetylneuraminic acid from the aqueous medium.

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Tokyo, Japan

Kazuhiko Tabata