Kikkoman Corporation

Kazuhiko Shimoji

Atsushi Ichiyanagi

Kozo Hirokawa

Keisuke Furukawa

Eriko Yoshihara

To reduce the effect of L-proline in the reaction of a sarcosine oxidase. A modified sarcosine oxidase having reduced reactivity to L-proline is provided.

Modified sarcosine oxidase, and gene and production method therefor

Provided is a compound which accelerates the enzymatic reaction catalyzed by an oxidase. The present invention provides an oxidase reaction accelerating agent comprising a compound represented by formula (I) and a method using the same.

Reaction accelerating agent

Provided is a compound capable of accelerating the enzymatic reaction catalyzed by peroxidase. The present invention provides a peroxidase reaction accelerating agent comprising a compound represented by formula (I) and a method for measuring hydrogen peroxide using the same.

The present invention relates to a method for quantitative determination of an &#x3b1;-glycated peptide in a sample, comprising causing protease to act on a whole blood and/or blood cell sample, causing an elimination reagent containing one or a plurality of types of ketoamine oxidase to act on the resultant, eliminating an &#x3b1;-glycated amino acid, an &#x3b5;-glycated amino acid, an &#x3b5;-glycated peptide, or a combination thereof, and then determining the &#x3b1;-glycated peptide in the sample using oxidase that acts on the &#x3b1;-glycated peptide. The present invention also relates to an elimination reagent and a kit to be used for such method. According to the present invention, measurement errors in quantitative determination of a glycated protein such as glycated hemoglobin can be reduced, and thus a precise measured value can be obtained.

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Chiba, Japan

Kazuhiko Shimoji