Sekisui Chemical Co., Ltd.

Kazuhiko Shimada

Kazuyuki Oishi

Toshiki Kawabe

Yuji Setoguchi

Takayuki Oka

Masaaki Minamoto

The present invention provides a blood coagulation accelerator excellent under severe conditions at ordinary or higher temperatures and capable of exhibiting high blood coagulation performance stably over a long period of time, as well as a container for blood examination wherein the blood coagulation accelerator is accommodated. Disclosed is a blood coagulation accelerator comprising an enzyme capable of hydrolyzing in a peptide chain a bond between arginine and an arbitrary amino acid residue and/or a bond between lysine and an arbitrary amino acid residue, an inactivated enzyme product comprising the above-mentioned enzyme inactivated by radiation irradiation, and &#x3b2;-alanine.

Blood coagulation accelerator and container for blood examination

A method for separating hemoglobin A from a hemoglobin mixture according to a cation exchange liquid chromatography characterized that at least two types of eluents are used including an eluent (hereinafter referred to as &ldquo;eluent A&rdquo;) for elution of faster hemoglobins than hemoglobin A and an eluent (hereinafter referred to as &ldquo;eluent B&rdquo;) for elution of hemoglobin A and slower hemoglobins than hemoglobin A , and that the eluent A has a pH of 4.0-6.0 and the eluent B is maintained at a pH of at least 0.5 higher than that of the eluent A.

Method of separating hemoglobin A2

A method for determining hemoglobins is provided which is suitable for determination of stable hemoglobin A . A method for determining hemoglobins by cation exchange liquid chromatography wherein an eluent is used containing a chaotropic ion and further an inorganic acid, an organic acid and/or any salt thereof having a buffer capacity in the 4.0-6.8 pH range.

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Tokuyama, Japan

Kazuhiko Shimada